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Literature summary for 3.6.4.B7 extracted from

  • Patoli, B.B.; Winter, J.A.; Patoli, A.A.; Delahay, R.M.; Bunting, K.A.
    Co-expression and purification of the RadA recombinase with the RadB paralog from Haloferax volcanii yields heteromeric ring-like structures (2017), Microbiology, 163, 1802-1811 .
    View publication on PubMed
Search for more literature for 3.6.4.B7

Cloned(Commentary)

Cloned (Comment) Organism
gene radA, recombinant coexpression of His-tagged RadA with untagged RadB (UniProt ID D4GZB4), which form the RadAB complex, in Escherichia coli strain Rosetta2 (DE3) with deleted gene arnA. The ratio of RadA to RadB is 4:1, which suggests that the proteins interact with a specific stoichiometry Haloferax volcanii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Haloferax volcanii
-
 ADP + phosphate
-
 ?
ATP + H2O Haloferax volcanii NCIMB 2012
-
 ADP + phosphate
-
 ?
ATP + H2O Haloferax volcanii JCM 8879
-
 ADP + phosphate
-
 ?
ATP + H2O Haloferax volcanii DS2
-
 ADP + phosphate
-
 ?
ATP + H2O Haloferax volcanii DSM 3757
-
 ADP + phosphate
-
 ?
ATP + H2O Haloferax volcanii ATCC 29605
-
 ADP + phosphate
-
 ?
ATP + H2O Haloferax volcanii NBRC 14742
-
 ADP + phosphate
-
 ?
ATP + H2O Haloferax volcanii VKM B-1768
-
 ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Haloferax volcanii Q48328
-
-
Haloferax volcanii ATCC 29605 Q48328
-
-
Haloferax volcanii DS2 Q48328
-
-
Haloferax volcanii DSM 3757 Q48328
-
-
Haloferax volcanii JCM 8879 Q48328
-
-
Haloferax volcanii NBRC 14742 Q48328
-
-
Haloferax volcanii NCIMB 2012 Q48328
-
-
Haloferax volcanii VKM B-1768 Q48328
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant copurification of the RadAB complex from Escherichia coli strain Rosetta2 (DE3) DELTAarnA by cobalt affinity chromatography and gel filtration Haloferax volcanii

Source Tissue

Source Tissue Comment Organism Textmining
additional information Haloferax volcanii requires 2 M sodium chloride to maintain internal osmotic balance in artificial media Haloferax volcanii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Haloferax volcanii ADP + phosphate
-
 ?
ATP + H2O
-
 Haloferax volcanii NCIMB 2012 ADP + phosphate
-
 ?
ATP + H2O
-
 Haloferax volcanii JCM 8879 ADP + phosphate
-
 ?
ATP + H2O
-
 Haloferax volcanii DS2 ADP + phosphate
-
 ?
ATP + H2O
-
 Haloferax volcanii DSM 3757 ADP + phosphate
-
 ?
ATP + H2O
-
 Haloferax volcanii ATCC 29605 ADP + phosphate
-
 ?
ATP + H2O
-
 Haloferax volcanii NBRC 14742 ADP + phosphate
-
 ?
ATP + H2O
-
 Haloferax volcanii VKM B-1768 ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
More RadA multimerizes into a ring-like structure in the absence of DNA and nucleoside cofactor Haloferax volcanii

Synonyms

Synonyms Comment Organism
Hvo RadA
-
 Haloferax volcanii
RadA recombinase
-
 Haloferax volcanii

General Information

General Information Comment Organism
additional information homology modeling of the Hvo RadA primary sequence using the Pfu RadA structure (PDB ID 1PZN) chain A as the highest scoring template. Consistent with its function as a recombinase, ATP and DNA binding motifs are apparent which have a well-conserved sequence composition despite the common skews in overall amino acid usage typically observed in halophilic proteins Haloferax volcanii