Cloned (Comment) | Organism |
---|---|
gene radA, recombinant overexpression in Escherichia coli strain Rosetta | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Saccharolobus solfataricus | - |
ADP + phosphate | - |
? | |
ATP + H2O | Saccharolobus solfataricus P2 | - |
ADP + phosphate | - |
? | |
ATP + H2O | Saccharolobus solfataricus JCM 11322 | - |
ADP + phosphate | - |
? | |
ATP + H2O | Saccharolobus solfataricus ATCC 35092 | - |
ADP + phosphate | - |
? | |
ATP + H2O | Saccharolobus solfataricus DSM 1617 | - |
ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q55075 | i.e. Sulfolobus solfataricus | - |
Saccharolobus solfataricus ATCC 35092 | Q55075 | i.e. Sulfolobus solfataricus | - |
Saccharolobus solfataricus DSM 1617 | Q55075 | i.e. Sulfolobus solfataricus | - |
Saccharolobus solfataricus JCM 11322 | Q55075 | i.e. Sulfolobus solfataricus | - |
Saccharolobus solfataricus P2 | Q55075 | i.e. Sulfolobus solfataricus | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain Rosetta by affinity and anion exchange chromatography, and dialysis, protein concentration using PEG, method, overview | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Saccharolobus solfataricus | ADP + phosphate | - |
? | |
ATP + H2O | - |
Saccharolobus solfataricus P2 | ADP + phosphate | - |
? | |
ATP + H2O | - |
Saccharolobus solfataricus JCM 11322 | ADP + phosphate | - |
? | |
ATP + H2O | - |
Saccharolobus solfataricus ATCC 35092 | ADP + phosphate | - |
? | |
ATP + H2O | - |
Saccharolobus solfataricus DSM 1617 | ADP + phosphate | - |
? | |
additional information | methods for DNA and ATP binding assays, strand invasion and exchange, and ATPase assays, detailed overview | Saccharolobus solfataricus | ? | - |
- |
|
additional information | methods for DNA and ATP binding assays, strand invasion and exchange, and ATPase assays, detailed overview | Saccharolobus solfataricus P2 | ? | - |
- |
|
additional information | methods for DNA and ATP binding assays, strand invasion and exchange, and ATPase assays, detailed overview | Saccharolobus solfataricus JCM 11322 | ? | - |
- |
|
additional information | methods for DNA and ATP binding assays, strand invasion and exchange, and ATPase assays, detailed overview | Saccharolobus solfataricus ATCC 35092 | ? | - |
- |
|
additional information | methods for DNA and ATP binding assays, strand invasion and exchange, and ATPase assays, detailed overview | Saccharolobus solfataricus DSM 1617 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 35900, about, sequence calculation | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
RadA | - |
Saccharolobus solfataricus |
RadA recombinase | - |
Saccharolobus solfataricus |
SsoRadA | - |
Saccharolobus solfataricus |
SsoRadA recombinase | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 80 | - |
Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharolobus solfataricus |