Literature summary for 3.6.4.B7 extracted from

Komori, K.; Miyata, T.; DiRuggiero, J.; Holley-Shanks, R.; Hayashi, I.; Cann, I.K.; Mayanagi, K.; Shinagawa, H.; Ishino, Y.
Both RadA and RadB are involved in homologous recombination in Pyrococcus furiosus (2000), J. Biol. Chem., 275, 33782-33790.

Search for more literature for 3.6.4.B7

Activating Compound

Activating Compound	Comment	Organism	Structure
ssDNA	ATPase activity of RadA is drastically stimulated by ssDNA and to a lesser extent by dsDNA. The activity increases linearly with ssDNA concentration until a saturation plateau, at which the apparent binding stoichiometry is 3 nucleotides for 1 RadA	Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38400	-	x * 38400, SDS-PAGE, calculated from sequence	Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O	Pyrococcus furiosus	DNA-dependent ATPase, D-loop formation, and strand exchange activities. The enzyme is involved in homologous recombination	ADP + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus furiosus	O74036	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O	DNA-dependent ATPase, D-loop formation, and strand exchange activities. The enzyme is involved in homologous recombination	Pyrococcus furiosus	ADP + phosphate	-	?
ATP + H2O	DNA-dependent ATPase, D-loop formation, and strand exchange activities	Pyrococcus furiosus	ADP + phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 38400, SDS-PAGE, calculated from sequence	Pyrococcus furiosus

Synonyms

Synonyms	Comment	Organism
RadA	-	Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
70	-	assay at	Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0017	-	ATP	pH 7.5, 70°C	Pyrococcus furiosus
0.017	-	ATP	pH 7.5, 70°C, addition of dsDNA	Pyrococcus furiosus
0.0345	-	ATP	pH 7.5, 70°C, addition of ssDNA	Pyrococcus furiosus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Pyrococcus furiosus

Expression

Organism	Comment	Expression
Pyrococcus furiosus	expression of radA might be constitutive	additional information

General Information

General Information	Comment	Organism
physiological function	the enzyme is involved in homologous recombination	Pyrococcus furiosus

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Release 2023.1 (January 2023)