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Literature summary for 3.6.4.B7 extracted from

  • Graham, W.J.; Haseltine, C.A.
    A recombinase paralog from the hyperthermophilic crenarchaeon Sulfolobus solfataricus enhances SsoRadA ssDNA binding and strand displacement (2013), Gene, 515, 128-139.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
SsoRal3 UniProtID Q97X93, pI of 6.7, a recombinase paralogue from Sulfolobus solfataricus that enhances SsoRadA ssDNA binding and strand displacement. SsoRal3 protein is a ssDNA-dependent ATPase that can catalyze strand invasion at both saturating and subsaturating concentrations. It can bind both ssDNA and dsDNA, but its binding preference is altered by the presence or absence of ATP. SsoRal3 alters SsoRadA ssDNA-dependent ATPase activity, addition of SsoRal3 to SsoRadA nucleoprotein filaments reduces total ATPase activity. Subsaturating concentrations of SsoRal3 increase the ssDNA binding activity of SsoRadA approximately 9fold and also increase the persistence of SsoRadA catalyzed strand invasion products. SsoRal3 functions to stabilize the SsoRadA presynaptic filament. SsoRal3 can catalyze strand invasion and extends the persistence of SsoRadA-mediated D-loops Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment) Organism
gene radA, recombinant expression in Escherichia coli strain Rosetta Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Saccharolobus solfataricus
-
ADP + phosphate
-
?
ATP + H2O Saccharolobus solfataricus P2
-
ADP + phosphate
-
?
additional information Saccharolobus solfataricus formation of SsoRadA-ssDNA complexes resulting in SsoRadA nucleoprotein filaments ?
-
?
additional information Saccharolobus solfataricus P2 formation of SsoRadA-ssDNA complexes resulting in SsoRadA nucleoprotein filaments ?
-
?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q55075 gene radA
-
Saccharolobus solfataricus P2 Q55075 gene radA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain Rosetta by affinity chromatography, dialysis, anion exchange cchromatography Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
Saccharolobus solfataricus ADP + phosphate
-
?
ATP + H2O SsoRadA ssDNA-dependent ATPase activity Saccharolobus solfataricus ADP + phosphate
-
?
ATP + H2O
-
Saccharolobus solfataricus P2 ADP + phosphate
-
?
ATP + H2O SsoRadA ssDNA-dependent ATPase activity Saccharolobus solfataricus P2 ADP + phosphate
-
?
additional information formation of SsoRadA-ssDNA complexes resulting in SsoRadA nucleoprotein filaments Saccharolobus solfataricus ?
-
?
additional information formation of SsoRadA-ssDNA complexes Saccharolobus solfataricus ?
-
?
additional information formation of SsoRadA-ssDNA complexes resulting in SsoRadA nucleoprotein filaments Saccharolobus solfataricus P2 ?
-
?
additional information formation of SsoRadA-ssDNA complexes Saccharolobus solfataricus P2 ?
-
?

Synonyms

Synonyms Comment Organism
SsoRadA
-
Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
ATPase assay at Saccharolobus solfataricus
8
-
displacement loop assay at Saccharolobus solfataricus