Activating Compound | Comment | Organism | Structure |
---|---|---|---|
SsoRal3 | UniProtID Q97X93, pI of 6.7, a recombinase paralogue from Sulfolobus solfataricus that enhances SsoRadA ssDNA binding and strand displacement. SsoRal3 protein is a ssDNA-dependent ATPase that can catalyze strand invasion at both saturating and subsaturating concentrations. It can bind both ssDNA and dsDNA, but its binding preference is altered by the presence or absence of ATP. SsoRal3 alters SsoRadA ssDNA-dependent ATPase activity, addition of SsoRal3 to SsoRadA nucleoprotein filaments reduces total ATPase activity. Subsaturating concentrations of SsoRal3 increase the ssDNA binding activity of SsoRadA approximately 9fold and also increase the persistence of SsoRadA catalyzed strand invasion products. SsoRal3 functions to stabilize the SsoRadA presynaptic filament. SsoRal3 can catalyze strand invasion and extends the persistence of SsoRadA-mediated D-loops | Saccharolobus solfataricus |
Cloned (Comment) | Organism |
---|---|
gene radA, recombinant expression in Escherichia coli strain Rosetta | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Saccharolobus solfataricus | - |
ADP + phosphate | - |
? | |
ATP + H2O | Saccharolobus solfataricus P2 | - |
ADP + phosphate | - |
? | |
additional information | Saccharolobus solfataricus | formation of SsoRadA-ssDNA complexes resulting in SsoRadA nucleoprotein filaments | ? | - |
? | |
additional information | Saccharolobus solfataricus P2 | formation of SsoRadA-ssDNA complexes resulting in SsoRadA nucleoprotein filaments | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q55075 | gene radA | - |
Saccharolobus solfataricus P2 | Q55075 | gene radA | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain Rosetta by affinity chromatography, dialysis, anion exchange cchromatography | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Saccharolobus solfataricus | ADP + phosphate | - |
? | |
ATP + H2O | SsoRadA ssDNA-dependent ATPase activity | Saccharolobus solfataricus | ADP + phosphate | - |
? | |
ATP + H2O | - |
Saccharolobus solfataricus P2 | ADP + phosphate | - |
? | |
ATP + H2O | SsoRadA ssDNA-dependent ATPase activity | Saccharolobus solfataricus P2 | ADP + phosphate | - |
? | |
additional information | formation of SsoRadA-ssDNA complexes resulting in SsoRadA nucleoprotein filaments | Saccharolobus solfataricus | ? | - |
? | |
additional information | formation of SsoRadA-ssDNA complexes | Saccharolobus solfataricus | ? | - |
? | |
additional information | formation of SsoRadA-ssDNA complexes resulting in SsoRadA nucleoprotein filaments | Saccharolobus solfataricus P2 | ? | - |
? | |
additional information | formation of SsoRadA-ssDNA complexes | Saccharolobus solfataricus P2 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SsoRadA | - |
Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
ATPase assay at | Saccharolobus solfataricus |
8 | - |
displacement loop assay at | Saccharolobus solfataricus |