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Literature summary for 3.6.4.B4 extracted from

  • Graham, W.J.; Haseltine, C.A.
    A recombinase paralog from the hyperthermophilic crenarchaeon Sulfolobus solfataricus enhances SsoRadA ssDNA binding and strand displacement (2013), Gene, 515, 128-139.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.4.B4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31300
-
 x * 31300, calculated from sequence Saccharolobus solfataricus
34300
-
 x * 34300, SDS-PAGE Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Saccharolobus solfataricus model for the involvement of SsoRal3 in stabilization of the SsoRadA presynaptic filament during strand invasion: SsoRadA presynaptic filament formation and strand invasion in the presence of SsoRal3. SsoRal3 is stored in an inactive state on dsDNA in the absence of ATP. ATP addition permits SsoRal3 binding to ssDNA, stabilizing the presynaptic filament and enhancing strand invasion ADP + phosphate
-
 ?
ATP + H2O Saccharolobus solfataricus P2 model for the involvement of SsoRal3 in stabilization of the SsoRadA presynaptic filament during strand invasion: SsoRadA presynaptic filament formation and strand invasion in the presence of SsoRal3. SsoRal3 is stored in an inactive state on dsDNA in the absence of ATP. ATP addition permits SsoRal3 binding to ssDNA, stabilizing the presynaptic filament and enhancing strand invasion ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97X93
-
-
Saccharolobus solfataricus P2 Q97X93
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Reaction

Reaction Comment Organism Reaction ID
ATP + H2O = ADP + phosphate the enzyme functions to stabilize the presynaptic filament Saccharolobus solfataricus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O model for the involvement of SsoRal3 in stabilization of the SsoRadA presynaptic filament during strand invasion: SsoRadA presynaptic filament formation and strand invasion in the presence of SsoRal3. SsoRal3 is stored in an inactive state on dsDNA in the absence of ATP. ATP addition permits SsoRal3 binding to ssDNA, stabilizing the presynaptic filament and enhancing strand invasion Saccharolobus solfataricus ADP + phosphate
-
 ?
ATP + H2O the enzyme shows almost no ATP hydrolysis in the absence of DNA. SsoRal3 can bind both ss- and dsDNA. The presence of ATP enhances the ability of SsoRal3 to bind ssDNA. The opposite effect is observed when dsDNA was used. When bound to either substrate, the strength of binding is nearly equivalent. SsoRal3 ssDNA binding stabilizes the SsoRadA-ssDNA nucleoprotein complex only when ATP is present Saccharolobus solfataricus ADP + phosphate
-
 ?
ATP + H2O model for the involvement of SsoRal3 in stabilization of the SsoRadA presynaptic filament during strand invasion: SsoRadA presynaptic filament formation and strand invasion in the presence of SsoRal3. SsoRal3 is stored in an inactive state on dsDNA in the absence of ATP. ATP addition permits SsoRal3 binding to ssDNA, stabilizing the presynaptic filament and enhancing strand invasion Saccharolobus solfataricus P2 ADP + phosphate
-
 ?
ATP + H2O the enzyme shows almost no ATP hydrolysis in the absence of DNA. SsoRal3 can bind both ss- and dsDNA. The presence of ATP enhances the ability of SsoRal3 to bind ssDNA. The opposite effect is observed when dsDNA was used. When bound to either substrate, the strength of binding is nearly equivalent. SsoRal3 ssDNA binding stabilizes the SsoRadA-ssDNA nucleoprotein complex only when ATP is present Saccharolobus solfataricus P2 ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 31300, calculated from sequence Saccharolobus solfataricus
? x * 34300, SDS-PAGE Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
SSO1861
-
 Saccharolobus solfataricus
SsoRal3 protein
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 80 60°C: about 40% of maximal activity, 80°C: about 40% of maximal activity Saccharolobus solfataricus