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Literature summary for 3.6.4.B10 extracted from

  • Gao, L.; Danno, A.; Fujii, S.; Fukuda, W.; Imanaka, T.; Fujiwara, S.
    Indole-3-glycerol-phosphate synthase is recognized by a cold-inducible group II chaperonin in Thermococcus kodakarensis (2012), Appl. Environ. Microbiol., 78, 3806-3815.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.4.B10

Cloned(Commentary)

Cloned (Comment) Organism
gene cpkA, recombinant expression of the chimeric chaperonin CpkBA in Escherichia coli Thermococcus kodakarensis
gene cpkB, recombinant expression of the chimeric chaperonin CpkBA in Escherichia coli Thermococcus kodakarensis

Protein Variants

Protein Variants Comment Organism
additional information construction of gene cpkA disruption mutant strains. Gene disruptant strain DA1 (DELTAcpkA) shows decreased cell growth at 60°C as compared to 85°C for the wild-type strain KOD1. The DB2 mutant (DELTAcpkA::cpkB DELTAcpkB), whose cpkB gene is expressed under the control of the cpkA promoter, does not grow at 60°C, and the DB3 mutant (DELTAcpkA(1-524)::cpkB(1-524) DELTAcpkB), whose CpkA amino acid residues 1 to 524 are replaced with corresponding CpkB residues that maintains the C-terminal region intact, grows at 60°C, implying that the CpkA C-terminal region plays a key role in cell growth at 60°C. Proteins coimmunoprecipitated with anti-Cpk from DB1 cells cultivated at 60°C, overview Thermococcus kodakarensis
additional information construction of gene cpkB disruption mutant strains. Gene disruptant strain DB1 (DELTAcpkB) shows decreased cell growth at 93°C as compared to 85°C for the wild-type strain KOD1. The DB2 mutant (DELTAcpkA::cpkB DELTAcpkB), whose cpkB gene is expressed under the control of the cpkA promoter, does not grow at 60°C, and the DB3 mutant (DELTAcpkA(1-524)::cpkB(1-524) DELTAcpkB), whose CpkA amino acid residues 1 to 524 are replaced with corresponding CpkB residues that maintains the C-terminal region intact, grows at 60°C, implying that the CpkA C-terminal region plays a key role in cell growth at 60°C. Proteins coimmunoprecipitated with anti-Cpk from DB1 cells cultivated at 60°C, overview Thermococcus kodakarensis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Thermococcus kodakarensis 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Thermococcus kodakarensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 60000, about, CpkA, SDS-PAGE Thermococcus kodakarensis
60000
-
 x * 60000, about, CpkB, SDS-PAGE Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Thermococcus kodakarensis
-
 ADP + phosphate
-
 ?
additional information Thermococcus kodakarensis indole-3-glycerol-phosphate synthase, TrpC, is a specific target protein of CpkA ?
-
 ?
additional information Thermococcus kodakarensis indole-3-glycerol-phosphate synthase, TrpC, is no target protein for CpkB ?
-
 ?

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis P61111 subunit alpha; cold-inducible CpkA
-
Thermococcus kodakarensis Q52500 subunit beta; heat-inducible CpkB
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information Thermococcus kodakarensis optimally grows at 85°C, gene disruptant strain DA1 (DELTAcpkA) shows decreased cell growth at 60°C Thermococcus kodakarensis
-
additional information Thermococcus kodakarensis optimally grows at 85°C, gene disruptant strain DB1 (DELTAcpkB) shows decreased cell growth at 93°C Thermococcus kodakarensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
 Thermococcus kodakarensis ADP + phosphate
-
 ?
additional information indole-3-glycerol-phosphate synthase, TrpC, is a specific target protein of CpkA Thermococcus kodakarensis ?
-
 ?
additional information indole-3-glycerol-phosphate synthase, TrpC, is no target protein for CpkB Thermococcus kodakarensis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, about, CpkA, SDS-PAGE Thermococcus kodakarensis
? x * 60000, about, CpkB, SDS-PAGE Thermococcus kodakarensis

Synonyms

Synonyms Comment Organism
cPKA
-
 Thermococcus kodakarensis
CpkB
-
 Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 ATPase assay at Thermococcus kodakarensis
8
-
 chaperonin assay at Thermococcus kodakarensis

General Information

General Information Comment Organism
malfunction gene disruptant strain DA1 (DELTAcpkA) shows decreased cell growth at 60°C, DA1 cells grow optimally in minimal medium only in the presence of tryptophan but hardly grow in the absence of tryptophan at 60°C. A lesion of functional indole-3-glycerol-phosphate synthase, TrpC, is caused by cpkA disruption, resulting in tryptophan auxotrophy Thermococcus kodakarensis
malfunction gene disruptant strain DB1 (DELTAcpkB) shows decreased cell growth at 93°C Thermococcus kodakarensis
physiological function the CpkA C-terminal region plays a key role in cell growth at 60°C Thermococcus kodakarensis