Cloned (Comment) | Organism |
---|---|
gene PEX1, functional expression of FLAG-tagged Pex1 in Saccharomyces cerevisiae, FLAG-tagged Pex1 and His-tagged Pex6 coexpression in Escherichia coli | Saccharomyces cerevisiae |
gene PEX6, functional expression of FLAG-tagged Pex6 in Saccharomyces cerevisiae, FLAG-tagged Pex1 and His-tagged Pex6 coexpression in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a mutation of the conserved Walker A lysine in the D1 domain of Pex1, but not Pex6, dramatically affects the recovery of fully assembled recombinant hexamer. Compared to the ATPase rate of wild-type Pex1/Pex6, this Pex1 D1 Walker A mutant hexamer retains 70% activity. The Pex1 and Pex6 D2 Walker B double mutant shows no ATP-hydrolysis activity. The ATPase rate of the Pex1-WB/Pex6 mutant is very similar to the rate observed for wild-type Pex1/Pex6 at saturating concentrations of tPex15, but unlike for wild-type Pex1/Pex6, no inhibition of Pex1-WB/Pex6 by tPex15 is observed | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | Pex1 is completely inhibited when Pex6 is trapped in a permanently ATP-bound state, whereas Pex6 maintains some hydrolysis activity despite an ATP-bound state in Pex1; Pex1 is completely inhibited when Pex6 is trapped in a permanently ATP-bound state, whereas Pex6 maintains some hydrolysis activity despite an ATP-bound state in Pex1 | Saccharomyces cerevisiae | |
Pex15 | the membrane anchor required for Pex1/Pex6 recruitment to peroxisomes inhibits the ATP-hydrolysis activity of Pex1/Pex6; the membrane anchor required for Pex1/Pex6 recruitment to peroxisomes inhibits the ATP-hydrolysis activity of Pex1/Pex6 | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
ATP | recombinant Pex1-FLAG/His-Pex6 complex, pH and temperature not specified in the publication | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisomal membrane | Pex15 is the membrane anchor required for Pex1/Pex6 recruitment to peroxisomes | Saccharomyces cerevisiae | 5778 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Saccharomyces cerevisiae | the recombinant Pex1-FLAG/His-Pex6 complex is an active ATPase | ADP + phosphate | - |
? | |
additional information | Saccharomyces cerevisiae | Pex1 and Pex6 interact in vivo in an ATP-dependent manner. In the absence of nucleotide, the association of Pex1 with Pex6-FLAG is substantially diminished | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P24004 | PEX1 | - |
Saccharomyces cerevisiae | P33760 | PEX6 | - |
Purification (Comment) | Organism |
---|---|
copurification of recombinant FLAG-tagged Pex1 and His-tagged Pex6 from Escherichia coli by tandem-affinity chromatography using Ni-NTA agarose and alpha-FLAG affinity resin, followed by gel filtration | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Saccharomyces cerevisiae | ADP + phosphate | - |
? | |
ATP + H2O | the recombinant Pex1-FLAG/His-Pex6 complex is an active ATPase | Saccharomyces cerevisiae | ADP + phosphate | - |
? | |
additional information | Pex1 and Pex6 interact in vivo in an ATP-dependent manner. In the absence of nucleotide, the association of Pex1 with Pex6-FLAG is substantially diminished | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterohexamer | the Pex1/Pex6 complex is a heterohexameric AAA+ motor with alternating and highly coordinated subunits, structure analysis, overview | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Pex1 | - |
Saccharomyces cerevisiae |
Pex1/Pex6 complex | - |
Saccharomyces cerevisiae |
PEX6 | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
evolution | enzymes Pex1 and Pex6 are type-2 AAA+ ATPases | Saccharomyces cerevisiae |
malfunction | a mutation of the conserved Walker A lysine in the D1 domain of Pex1, but not Pex6, dramatically affects the recovery of fully assembled recombinant hexamer | Saccharomyces cerevisiae |
additional information | within the Pex1/Pex6 complex, only the D2 ATPase ring hydrolyzes ATP, while nucleotide binding in the D1 ring promotes complex assembly. ATP hydrolysis by Pex1 is highly coordinated with that of Pex6 | Saccharomyces cerevisiae |
physiological function | Pex1 and Pex6 are required for the de novo biogenesis of peroxisomes. The Pex1/Pex6 complex is a heterohexameric AAA+ motor with alternating and highly coordinated subunits. The recombinant Pex1-FLAG/His-Pex6 complex is an active ATPase | Saccharomyces cerevisiae |