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Literature summary for 3.6.4.6 extracted from
- Pctaire1 phosphorylates N-ethylmaleimide-sensitive fusion protein: implications in the regulation of its hexamerization and exocytosis (2006), J. Biol. Chem., 281, 9852-9858.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| full-length, partial cDNA fragments and point mutations of mouse NSF are amplified by PCR and subcloned into different expression vectors including pGEX-6P-2, pETH-32, pcDNA3-His6, and pcDNA3-3HA | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S569A | mutation of serine 569 to alanine abolishs the phosphorylation of D2 domain by active Pctaire1 | Mus musculus |
| S569A | mutation of serine 569 to alanine abolishs the phosphorylation of D2 domain by active Pctaire1 | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | phosphorylation by Pctaire1 attenuates the hexamerization of NSF | Mus musculus | |
| additional information | phosphorylation by Pctaire1 attenuates the hexamerization of NSF | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Mus musculus | 5829 | - |
| cytosol | - |
Rattus norvegicus | 5829 | - |
| membrane | NSF requires a peripheral membrane protein to bind membranes | Mus musculus | 16020 | - |
| membrane | NSF requires a peripheral membrane protein to bind membranes | Rattus norvegicus | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Mus musculus | - |
ADP + phosphate | - |
ir |  |
| ATP + H2O | Rattus norvegicus | - |
ADP + phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | phosphorylated by Pctaire1 on serine 569 | Mus musculus |
| phosphoprotein | phosphorylated by Pctaire1 on serine 569 | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| protein extracts from cells and brain tissues are prepared | Rattus norvegicus |
| recombinant NSF is purified by nickel-nitrilotriacetic acid-agarose affinity chromatography | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| nervous system | - |
Rattus norvegicus | - |
| PC-12 cell | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Mus musculus | ADP + phosphate | - |
ir | |
| ATP + H2O | - |
Rattus norvegicus | ADP + phosphate | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | - |
Mus musculus |
| homohexamer | - |
Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| N-ethylmaleimide-sensitive fusion protein | - |
Mus musculus |
| N-ethylmaleimide-sensitive fusion protein | - |
Rattus norvegicus |
| NSF | - |
Mus musculus |
| NSF | - |
Rattus norvegicus |
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Release 2023.1 (January 2023)
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