Literature summary for 3.6.4.10 extracted from

Cai, K.; Frederick, R.O.; Kim, J.H.; Reinen, N.M.; Tonelli, M.; Markley, J.L.
Human mitochondrial chaperone (mtHSP70) and cysteine desulfurase (NFS1) bind preferentially to the disordered conformation, whereas co-chaperone (HSC20) binds to the structured conformation of the iron-sulfur cluster scaffold protein (ISCU) (2013), J. Biol. Chem., 288, 28755-28770.

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Activating Compound

Activating Compound	Comment	Organism	Structure
Hsc20	the ATPase activity of mtHSP70 is accelerated by co-chaperone HSC20 and further accelerated by HSC20 plus scaffold protein ISCU. mtHSP70 binds preferentially to the D-state of ISCU and that HSC20 binds preferentially to the S-state of ISCU	Homo sapiens
ISCU	the iron-sulfur cluster scaffold protein, the ATPase activity of mtHSP70 is accelerated by co-chaperone HSC20 and further accelerated by HSC20 plus scaffold protein ISCU. mtHSP70 binds preferentially to the D-state of ISCU and that HSC20 binds preferentially to the S-state of ISCU, detailed interaction analysis	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of His-tagged enzyme mtHSP70 as N-terminal SUMO fusion protein, lacking the gene sequence coding for N-terminal mitochondrial targeting peptide, in Escherichia coli	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Homo sapiens	5739	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O	Homo sapiens	-	ADP + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged SUMO fusion enzyme mtHSP70 from Escherichia coli by nickel affinity chromatography, His-tag and SUMO cleavage and again nickel affinity exchange chromatography for tag removal, followed by gel filtration	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.00167	-	purified recombinant enzyme, pH 7.5, 25°C, in absence of activators	Homo sapiens
0.00283	-	purified recombinant enzyme, pH 7.5, 25°C, in presence of 0.006 mM HSC20	Homo sapiens
0.0075	-	purified recombinant enzyme, pH 7.5, 25°C, in presence of 0.015 mM ISCU	Homo sapiens
0.025	-	purified recombinant enzyme, pH 7.5, 25°C, in presence of 0.004 mM HSC20 and 0.015 mM ISCU	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O	-	Homo sapiens	ADP + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
mitochondrial chaperone	-	Homo sapiens
mtHSP70	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Homo sapiens

General Information

General Information	Comment	Organism
metabolism	iron-sulfur cluster biosynthesis involves a scaffold protein (ISCU), cysteine desulfurase (NFS1), chaperone (mtHSP70), and co-chaperone (HSC20). The ATPase activity of mtHSP70 is accelerated by ch-chaperone HSC20 and further accelerated by HSC20 plus scaffold protein ISCU. mtHSP70 binds preferentially to the D-state of ISCU and that HSC20 binds preferentially to the S-state of ISCU	Homo sapiens

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Release 2023.1 (January 2023)