Cloned (Comment) | Organism |
---|---|
cloning from spleen cDNA, DNA and amino acid sequence determination and analysis, recombinant expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Paralichthys olivaceus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a mutant PoHsp70, PoHsp70M, that bears a mutation of the ATPase-associated domain, is completely abolished in activity. Construction of the DNA vaccine plasmids with wild-type and mutant enzymes, pSia10Hsp70 and pSia10Hsp70M. pSia10Hsp70 induces a survival rate that is significantly higher than that induced by pSia10, while pSia10Hsp70M induces a survival rate similar to that induced by pSia10 | Paralichthys olivaceus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Paralichthys olivaceus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Paralichthys olivaceus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70100 | - |
x * 70100, about, sequence calculation | Paralichthys olivaceus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Paralichthys olivaceus | - |
ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paralichthys olivaceus | O93240 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography | Paralichthys olivaceus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Paralichthys olivaceus | ADP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70100, about, sequence calculation | Paralichthys olivaceus |
More | all Hsp70 molecules possess a conserved architecture that consists of a 44 kDa N-terminal adenine nucleotide-binding domain (NBD), an 18 kDa substrate-binding domain (SBD), and a 10 kDa C-terminal domain (CTD) | Paralichthys olivaceus |
Synonyms | Comment | Organism |
---|---|---|
heat shock protein 70 | - |
Paralichthys olivaceus |
Hsp70 | - |
Paralichthys olivaceus |
PoHsp70 | - |
Paralichthys olivaceus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Paralichthys olivaceus | sequence calculation | - |
5.4 |
Organism | Comment | Expression |
---|---|---|
Paralichthys olivaceus | expression of PoHsp70 is upregulated in a time-dependent manner by vaccination of flounder with a DNA vaccine, pSia10, that expresses a Streptococcus iniae antigen Sia10 | up |
General Information | Comment | Organism |
---|---|---|
additional information | all Hsp70 molecules possess a conserved architecture that consists of a 44 kDa N-terminal adenine nucleotide-binding domain (NBD), which hydrolyzes ATP and thus is also called the ATPase domain, an 18 kDa substrate-binding domain (SBD) that contains a hydrophobic pocket in which direct substrate interaction occurs, and a 10 kDa C-terminal domain (CTD) that facilitates substrate trapping by forming a lid-like structure over the substrate binding pocket. PoHsp70 possesses an ATPase domain (residues 108e433) that contains the conserved residues (D201, S210, and G341) involved in ATP binding | Paralichthys olivaceus |
physiological function | heat shock protein 70 is a molecular chaperone that plays an important role in protein folding and transport. It is also involved in regulation of innate and adaptive immune response. PoHsp70 is an effective adjuvant and that the adjuvanticity of PoHsp70 requires the intrinsic ATPase activity | Paralichthys olivaceus |