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Literature summary for 3.6.4.10 extracted from

  • Li, J.; Wu, Y.; Qian, X.; Sha, B.
    Crystal structure of yeast Sis1 peptide-binding fragment and Hsp70 Ssa1 C-terminal complex (2006), Biochem. J., 398, 353-360.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of yeast Sis1 peptide-binding fragment and Hsp70 Ssa1 C-terminal complex, hanging drop vapour diffusion method, with the mother liquid containing 100 mM Tris/HCl, pH 7.0 and 42.5%(w/v) (NH4)2SO4. The crystals belong to the space group of P4(1)2(1)2 with the cell parameters of a = 112.17 A and c = 171.31 A Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
K199N missense Sis1 mutant exhibits greatly reduced binding affinity for the Ssa1 lid domain Saccharomyces cerevisiae
K202N missense Sis1 mutant exhibits greatly reduced binding affinity for the Ssa1 lid domain Saccharomyces cerevisiae
K214N missense Sis1 mutant exhibits greatly reduced binding affinity for the Ssa1 lid domain Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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Synonyms

Synonyms Comment Organism
Hsp40
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Saccharomyces cerevisiae
Hsp70
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Saccharomyces cerevisiae