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Literature summary for 3.6.4.10 extracted from
- ATPase activity and molecular chaperone function of the stress70 proteins (1996), Plant Physiol., 110, 419-424.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| p9/25PCR1 consists of a 2.4-kb cDNA encoding maize pre-stress70er cloned and expressed in Escherichia coli | Zea mays |
| pLeHsc70-2 consists of a 2.1-kb cDNA encoding tomato stress70c cloned into pUC19 | Solanum lycopersicum |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| 44-kDa wild-type stress70c ATPase fragment | Solanum lycopersicum |
| 44-kDa wild-type stress70er ATPase fragment | Zea mays |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Solanum lycopersicum | 5737 | - |
| endoplasmic reticulum | stress70 | Zea mays | 5783 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
- |
Homo sapiens |
| 44000 | - |
stable N-terminal ATPase fragment from native stress70, prepared by partial proteolysis | Zea mays |
| 44000 | - |
stable N-terminal ATPase fragment from native stress70, prepared by partial proteolysis | Solanum lycopersicum |
| 70000 | - |
- |
Escherichia coli |
| 70000 | - |
- |
Homo sapiens |
| 70000 | - |
- |
Zea mays |
| 70000 | - |
- |
Solanum lycopersicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Escherichia coli | - |
ADP + phosphate | - |
? |  |
| ATP + H2O | Homo sapiens | - |
ADP + phosphate | - |
? | |
| ATP + H2O | Zea mays | - |
ADP + phosphate | - |
? | |
| ATP + H2O | Solanum lycopersicum | - |
ADP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Homo sapiens | - |
human | - |
| Solanum lycopersicum | - |
tomato | - |
| Zea mays | - |
maize | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| both wild-type and recombinant proteins | Zea mays |
| both wild-type and recombinant proteins | Solanum lycopersicum |
| DnaK | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + H2O = ADP + phosphate | highly diverse group of enzymes that perform many functions that are similar to those of chaperonins. They comprise a number of heat-shock-cognate proteins. They are also active in clathrin uncoating and in the oligomerization of actin | Escherichia coli | |
| ATP + H2O = ADP + phosphate | highly diverse group of enzymes that perform many functions that are similar to those of chaperonins. They comprise a number of heat-shock-cognate proteins. They are also active in clathrin uncoating and in the oligomerization of actin | Homo sapiens | |
| ATP + H2O = ADP + phosphate | highly diverse group of enzymes that perform many functions that are similar to those of chaperonins. They comprise a number of heat-shock-cognate proteins. They are also active in clathrin uncoating and in the oligomerization of actin | Zea mays | |
| ATP + H2O = ADP + phosphate | highly diverse group of enzymes that perform many functions that are similar to those of chaperonins. They comprise a number of heat-shock-cognate proteins. They are also active in clathrin uncoating and in the oligomerization of actin | Solanum lycopersicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Escherichia coli | ADP + phosphate | - |
? | |
| ATP + H2O | - |
Homo sapiens | ADP + phosphate | - |
? | |
| ATP + H2O | - |
Zea mays | ADP + phosphate | - |
? | |
| ATP + H2O | - |
Solanum lycopersicum | ADP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Escherichia coli |
| dimer | - |
Homo sapiens |
| dimer | - |
Zea mays |
| dimer | - |
Solanum lycopersicum |
| monomer | - |
Zea mays |
| monomer | - |
Solanum lycopersicum |
| oligomer | - |
Escherichia coli |
| oligomer | - |
Zea mays |
| oligomer | - |
Solanum lycopersicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DnaK | - |
Escherichia coli |
| human stress70c | - |
Homo sapiens |
| maize stress70er | - |
Zea mays |
| stress70 protein | - |
Zea mays |
| stress70 protein | - |
Solanum lycopersicum |
| tomato stress70c | - |
Solanum lycopersicum |
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Release 2023.1 (January 2023)
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