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Literature summary for 3.6.1.B16 extracted from

  • Ito, D.; Kato, T.; Maruta, T.; Tamoi, M.; Yoshimura, K.; Shigeoka, S.
    Enzymatic and molecular characterization of Arabidopsis ppGpp pyrophosphohydrolase, AtNUDX26 (2012), Biosci. Biotechnol. Biochem., 76, 2236-2241.
    View publication on PubMed
Search for more literature for 3.6.1.B16

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.135
-
 guanosine 3',5'-bis(diphosphate) pH 8.0, 37°C, presence of 5 mM Mg2+ Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Arabidopsis thaliana 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ or Mn2+, absolutely required Arabidopsis thaliana
Mn2+ or Mg2+, absolutely required Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9CAF2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanosine 3',5'-bis(diphosphate) + 2 H2O
-
 Arabidopsis thaliana guanosine 3',5'-bisphoshate + 2 phosphate enzyme has diphosphohydrolase activity towards both diphosphate linkages in ppGpp and generates pGp as final product ?

Synonyms

Synonyms Comment Organism
Nudix hydrolase 26
-
 Arabidopsis thaliana
NUDX26
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.49
-
 guanosine 3',5'-bis(diphosphate) pH 8.0, 37°C, presence of 5 mM Mg2+ Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
7
-
 50% of maximum activity Arabidopsis thaliana
9.5
-
 50% of maximum activity Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana expression of isoform NUDX26 decreases upon treatment with paraquat and under salinity stress down

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6
-
 guanosine 3',5'-bis(diphosphate) pH 8.0, 37°C, presence of 5 mM Mg2+ Arabidopsis thaliana