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Literature summary for 3.6.1.B11 extracted from
- Schizosaccharomyces pombe Aps1, a diadenosine 5',5'''-P1, P6-hexaphosphate hydrolase that is a member of the nudix (MutT) family of hydrolases: cloning of the gene and characterization of the purified enzyme (1999), Biochemistry, 38, 3649-3655.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Schizosaccharomyces pombe |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.019 | - |
P1,P6-bis(5'-adenosyl)hexaphosphate | pH 7.6, 37°C | Schizosaccharomyces pombe |  |
| 0.022 | - |
P1,P5-bis(5'-adenosyl)pentaphosphate | pH 7.6, 37°C | Schizosaccharomyces pombe | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM CaCl2, 45% of the activity with 0.1-1 mM MnCl2 | Schizosaccharomyces pombe | |
| Co2+ | 1 mM CoCl2, 45% of the activity with 0.1-1 mM MnCl2 | Schizosaccharomyces pombe | |
| Mg2+ | 1 mM MgCl2, 45% of the activity with 0.1-1 mM MnCl2 | Schizosaccharomyces pombe | |
| Mn2+ | optimal hydrolysis of P1,P6-bis(5'-adenosyl)hexaphosphate in the presence of 0.1-1 mM MnCl2. Gradual decrease to about 1/3 maximum activity at 5 mM | Schizosaccharomyces pombe | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23724 | - |
1 * 23724, calculated from sequence | Schizosaccharomyces pombe |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| P1,P5-bis(5'-adenosyl)pentaphosphate + H2O | Schizosaccharomyces pombe | - |
ATP + ADP | - |
? | |
| P1,P6-bis(5'-adenosyl)hexaphosphate + H2O | Schizosaccharomyces pombe | - |
adenosine 5'-tetraphosphate + ADP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schizosaccharomyces pombe | Q09790 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Schizosaccharomyces pombe |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme has limited activity on P1,P4-bis(5'-adenosyl)tetraphosphate and negligible activity on P1,P3-bis(5'-adenosyl)triphosphate, ADP-ribose, and NADH. Aps1 catalyzes the hydrolysis of mononucleotides with decreasing activity in order from p5A to AMP | Schizosaccharomyces pombe | ? | - |
? | |
| P1,P5-bis(5'-adenosyl)pentaphosphate + H2O | - |
Schizosaccharomyces pombe | ATP + ADP | - |
? | |
| P1,P5-bis(5'-adenosyl)pentaphosphate + H2O | P1,P6-bis(5'-adenosyl)hexaphosphate and P1,P5-bis(5'-adenosyl)pentaphosphate being the preferred substrates. 88.7% of the activity with P1,P6-bis(5'-adenosyl)hexaphosphate | Schizosaccharomyces pombe | ATP + ADP | - |
? | |
| P1,P6-bis(5'-adenosyl)hexaphosphate + H2O | - |
Schizosaccharomyces pombe | adenosine 5'-tetraphosphate + ADP | - |
? | |
| P1,P6-bis(5'-adenosyl)hexaphosphate + H2O | P1,P6-bis(5'-adenosyl)hexaphosphate and P1,P5-bis(5'-adenosyl)pentaphosphate being the preferred substrates | Schizosaccharomyces pombe | adenosine 5'-tetraphosphate + ADP | P1,P4-bis(5'-adenosyl)tetraphosphate and ADP are the major products. ATP is a minor product resulting from a direct, symmetrical cleavage of P1,P6-bis(5'-adenosyl)hexaphosphate. No hydrolysis of P1,P6-bis(5'-adenosyl)hexaphosphate to P1,P6-bis(5'-adenosyl)pentaphosphate and AMP. Most of the AMP present in this reaction must arise from the hydrolysis of the initial reaction products | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 23724, calculated from sequence | Schizosaccharomyces pombe |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| APS1 | - |
Schizosaccharomyces pombe |
| diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase | - |
Schizosaccharomyces pombe |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.7 | - |
P1,P5-bis(5'-adenosyl)pentaphosphate | pH 7.6, 37°C | Schizosaccharomyces pombe | |
| 2 | - |
P1,P6-bis(5'-adenosyl)hexaphosphate | pH 7.6, 37°C | Schizosaccharomyces pombe | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
hydrolysis of P1,P6-bis(5'-adenosyl)hexaphosphate | Schizosaccharomyces pombe |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.1 | 8.1 | pH 6.1: about 60% of maximal activity, pH 8.1: about 35% of maximal activity | Schizosaccharomyces pombe |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Schizosaccharomyces pombe | calculated from sequence | - |
9.49 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 77 | - |
P1,P5-bis(5'-adenosyl)pentaphosphate | pH 7.6, 37°C | Schizosaccharomyces pombe | |
| 105 | - |
P1,P6-bis(5'-adenosyl)hexaphosphate | pH 7.6, 37°C | Schizosaccharomyces pombe | |
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