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Literature summary for 3.6.1.72 extracted from

  • Chauleau, M.; Jacewicz, A.; Shuman, S.
    DNA3pp5G de-capping activity of aprataxin: effect of cap nucleoside analogs and structural basis for guanosine recognition (2015), Nucleic Acids Res., 43, 6075-6083.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.72

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure in complex with GMP, to 1.5 A resolution. GMP binds at the same position and in the same anti nucleoside conformation as AMP, and aprataxin makes more extensive nucleobase contacts with guanine than with adenine, via a hydrogen bonding network to the guanine O6, N1, N2 base edge Schizosaccharomyces pombe

Protein Variants

Protein Variants Comment Organism
D63A mutation impairs DNAppG decapping Schizosaccharomyces pombe
His147A mutation abolishes DNAppG decapping activity Schizosaccharomyces pombe
His149A mutation abolishes DNAppG decapping activity Schizosaccharomyces pombe

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe O74859
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-
Schizosaccharomyces pombe ATCC 24843 O74859
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information aprataxin is a DNA 3'-de-capping enzyme, converting DNAppG to DNA3'p and GMP. Aprataxin hydrolyzes inosine and 6-O-methylguanosine caps, but not adeoxyguanosine cap. The enzyme also possesses the activity of EC 3.1.11.7, adenosine-5'-diphospho-5'-[DNA] diphosphatase, and EC 3.1.11.8, guanosine-5'-diphospho-5'-[DNA] diphosphatase Schizosaccharomyces pombe ?
-
 ?
additional information aprataxin is a DNA 3'-de-capping enzyme, converting DNAppG to DNA3'p and GMP. Aprataxin hydrolyzes inosine and 6-O-methylguanosine caps, but not adeoxyguanosine cap. The enzyme also possesses the activity of EC 3.1.11.7, adenosine-5'-diphospho-5'-[DNA] diphosphatase, and EC 3.1.11.8, guanosine-5'-diphospho-5'-[DNA] diphosphatase Schizosaccharomyces pombe ATCC 24843 ?
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 ?
[DNA]-3'-diphospho-5'-(6-O-methylguanosine) + H2O
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 Schizosaccharomyces pombe [DNA]-3'-phosphate + 6-O-methyl-GMP
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 ?
[DNA]-3'-diphospho-5'-(6-O-methylguanosine) + H2O
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 Schizosaccharomyces pombe ATCC 24843 [DNA]-3'-phosphate + 6-O-methyl-GMP
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 ?
[DNA]-3'-diphospho-5'-guanosine + H2O
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 Schizosaccharomyces pombe [DNA]-3'-phosphate + GMP
-
 ?
[DNA]-3'-diphospho-5'-guanosine + H2O
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 Schizosaccharomyces pombe ATCC 24843 [DNA]-3'-phosphate + GMP
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 ?
[DNA]-3'-diphospho-5'-inosine + H2O
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 Schizosaccharomyces pombe [DNA]-3'-phosphate + IMP
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 ?

Synonyms

Synonyms Comment Organism
aprataxin
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 Schizosaccharomyces pombe
HNT3
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 Schizosaccharomyces pombe