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Literature summary for 3.6.1.7 extracted from
- Expression, purification, and characterization of acylphosphatase muscular isoenzyme as fusion protein with glutathione S-transferase (1995), Protein Expr. Purif., 6, 799-805.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| muscular isoenzyme as fusion protein with glutathione S-transferase, synthetic gene linked to the gene for glutathione S-transferase, expression in Escherichia coli strains DB1035 and TB1 | Homo sapiens |
General Stability
| General Stability | Organism |
|---|---|
| muscular enzyme, sensitive to urea: urea denaturation | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | phosphate: competitive inhibition, muscular enzyme, native Ki: 2.29 mM, recombinant, expressed as fusion protein with glutathione S-transferase, Ki: 3.05 | Homo sapiens |  |
| Urea | 2.7 M: 50% inhibition, 8M: 100% inhibition, 90% of its original activity regained in the absence of urea | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic data | Homo sapiens | |
| 0.85 | - |
benzoyl phosphate | muscular enzyme recombinant, expressed as fusion protein with glutathione S-transferase | Homo sapiens | |
| 1.53 | - |
benzoyl phosphate | muscular enzyme | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Homo sapiens | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 11360 | - |
muscle, calculated from amino acid sequence | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
human | - |
| Homo sapiens | - |
two different isoenzymes: skeletal muscle and erythrocyte enzyme | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | muscular isoenzyme, acetylated at the N-terminus | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| muscular isoenzyme as fusion protein with glutathione S-transferase, subsequently cleaved to fully active acylphosphatase and further purified | Homo sapiens |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| renaturation after thermal denaturation, 65°C | Homo sapiens |
| renaturation after treatment with urea | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4500 | - |
muscular isoenzyme | Homo sapiens |
| 5000 | - |
muscular isoenzyme, recombinant, expressed as fusion protein with glutathione S-transferase | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,3-diphosphoglycerate + H2O | - |
Homo sapiens | 3-phosphoglycerate + phosphate | - |
? | |
| acylphosphate + H2O | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Homo sapiens | carboxylate + phosphate | - |
? | |
| benzoyl phosphate + H2O | - |
Homo sapiens | benzoate + phosphate | - |
? | |
| carbamoyl phosphate + H2O | - |
Homo sapiens | carbamate + phosphate | - |
? | |
| additional information | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Homo sapiens | ? | - |
? | |
| additional information | hydrolyzes the beta-aspartylphosphate formed during the action of membrane pumps | Homo sapiens | ? | - |
? | |
| succinoyl phosphate + H2O | - |
Homo sapiens | succinate + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | two isoenzymatic forms: one prevalent in skeletal and cardiac muscle named muscle type, the other in red blood cells named erythrocyte or common type, even if both isoforms are expressed in all tissues | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
muscular isoenzyme, fully denatured protein. Thermal denaturation is reversibel | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
muscle enzyme is very basic protein | Homo sapiens |
| 4.7 | 5.6 | muscular enzyme | Homo sapiens |
| 4.8 | 5.8 | muscular enzyme, recombinant, expressed as fusion protein with glutathione S-transferase | Homo sapiens |
| 5.3 | - |
assay at | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2700 | - |
Urea | - |
Homo sapiens | |
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