Activating Compound | Comment | Organism | Structure |
---|---|---|---|
inosinic acid | brain enzyme, no effect up to 2.3 mM, 4.6 mM: 33% activation, further increase in concentration, does not produce further activation | Bos taurus | |
papain | brain enzyme, slight, 15%, activation | Bos taurus |
General Stability | Organism |
---|---|
brain enzyme, stable to proteolytic enzymes | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adrenaline | brain enzyme, at high concentrations, 12.5 mM: 65% inhibition | Bos taurus | |
dinitrophenol | brain enzyme, 0.25 mM: 50% inhibition | Bos taurus | |
guanidine | brain enzyme, 5 M: 100% reversible inhibition, 95% of original activity is recovered upon lowering the guanidine concentration by dilution | Bos taurus | |
additional information | brain enzyme, no inhibition by caffeine, inosinic acid, 0.5% trypsin and 1% papain | Bos taurus | |
Pepsin | brain enzyme, 2%: inactivation | Bos taurus | |
phosphate | brain enzyme 20 mM: 50-60% inhibition, 40 mM: 70-80% inhibition | Bos taurus | |
thyroxine | brain enzyme, more inhibitory when preincubation is conducted at 16°C than at 25°C | Bos taurus | |
Urea | brain enzyme, 5 M: 100% reversible inhibition, 95% of original activity is recovered upon lowering the urea concentration by dilution | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13200 | - |
brain, ultracentrifugation | Bos taurus |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | unstable to | Bos taurus |
Methanol | unstable to | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
bovine | - |
Purification (Comment) | Organism |
---|---|
brain | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2638 | - |
brain | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl phosphate + H2O | - |
Bos taurus | acetate + phosphate | - |
? | |
acylphosphate + H2O | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Bos taurus | carboxylate + phosphate | - |
? | |
carbamoyl phosphate + H2O | - |
Bos taurus | carbamate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | brain enzyme, 1 * 13200, ultracentrifugation | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | - |
assay at | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme is thermostable | Bos taurus |
37 | - |
brain enzyme, purified and dialyzed enzyme, 20 min, 27% inactivation, undialyzed enzyme: no inactivation | Bos taurus |
80 | - |
brain enzyme, partially purified, 15 min, pH 1: 100% activity retained, pH 7: 80-90% activity retained | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Bos taurus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
brain enzyme, stable over a wide pH range | Bos taurus |
1.5 | 8.2 | brain enzyme, 24 h: no decrease of activity | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
dinitrophenol | brain enzyme | Bos taurus |