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Literature summary for 3.6.1.7 extracted from
- Purification and properties of porcine testis acylphosphatase (1987), J. Biochem., 102, 1405-1414.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.16 | - |
benzoyl phosphate | - |
Sus scrofa |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 10600 | - |
sedimentation equilibrium | Sus scrofa |
| 11000 | - |
PAGE and SDS-PAGE | Sus scrofa |
| 11120 | - |
muscle, calculated from amino acid composition | Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,3-diphosphoglycerate + H2O | Gallus gallus | - |
3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | Sus scrofa | possible physiological substrate | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | Equus caballus | possible physiological substrate | 3-phosphoglycerate + phosphate | - |
? | |
| carbamoyl phosphate + H2O | Sus scrofa | possible physiological substrate | carbamate + phosphate | - |
? | |
| carbamoyl phosphate + H2O | Equus caballus | possible physiological substrate | carbamate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Equus caballus | - |
- |
- |
| Gallus gallus | - |
- |
- |
| Sus scrofa | - |
two different isozymes in testis and skeletal muscle | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no modification | - |
Gallus gallus |
| no modification | amount of sugar is insignificant | Sus scrofa |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| testis and skeletal muscle | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | skeletal muscle | Gallus gallus | - |
| muscle | skeletal muscle | Sus scrofa | - |
| muscle | skeletal muscle | Equus caballus | - |
| testis | - |
Sus scrofa | - |
| testis | - |
Equus caballus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7200 | - |
skeletal muscle, substrate benzoyl phosphate, 25°C | Sus scrofa |
| 10800 | - |
testis, substrate benzoyl phosphate, 25°C | Sus scrofa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, long-time stable | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,3-diphosphoglycerate + H2O | - |
Gallus gallus | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | - |
Sus scrofa | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | - |
Equus caballus | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | possible physiological substrate | Sus scrofa | 3-phosphoglycerate + phosphate | - |
? | |
| 1,3-diphosphoglycerate + H2O | possible physiological substrate | Equus caballus | 3-phosphoglycerate + phosphate | - |
? | |
| acylphosphate + H2O | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Gallus gallus | carboxylate + phosphate | - |
? | |
| acylphosphate + H2O | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Sus scrofa | carboxylate + phosphate | - |
? | |
| acylphosphate + H2O | specifically catalyzes the hydrolysis of the carboxyl-phosphate bond of various acylphosphates | Equus caballus | carboxylate + phosphate | - |
? | |
| benzoyl phosphate + H2O | - |
Gallus gallus | benzoate + phosphate | - |
? | |
| benzoyl phosphate + H2O | - |
Sus scrofa | benzoate + phosphate | - |
? | |
| benzoyl phosphate + H2O | - |
Equus caballus | benzoate + phosphate | - |
? | |
| carbamoyl phosphate + H2O | - |
Sus scrofa | carbamate + phosphate | - |
? | |
| carbamoyl phosphate + H2O | - |
Equus caballus | carbamate + phosphate | - |
? | |
| carbamoyl phosphate + H2O | possible physiological substrate | Sus scrofa | carbamate + phosphate | - |
? | |
| carbamoyl phosphate + H2O | possible physiological substrate | Equus caballus | carbamate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Gallus gallus |
| dimer | - |
Equus caballus |
| dimer | 2 * 11000, skeletal muscle, SDS-PAGE | Sus scrofa |
| monomer | - |
Gallus gallus |
| monomer | - |
Equus caballus |
| monomer | 1 * 11000, testis, SDS-PAGE | Sus scrofa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ch1 | multiple forms of chicken acylphosphatase, 2 isozymes: Ch1 and Ch2 differ in molecular weight, amino acid composition and kinetic parameters | Gallus gallus |
| Ch2 | multiple forms of chicken acylphosphatase, 2 isozymes: Ch1 and Ch2 differ in molecular weight, amino acid composition and kinetic parameters | Gallus gallus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Gallus gallus |
| 25 | - |
assay at | Sus scrofa |
| 25 | - |
assay at | Equus caballus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Gallus gallus |
| additional information | - |
- |
Equus caballus |
| additional information | - |
pI testis enzyme: 8.3, pI muscle enzyme: 10.6 | Sus scrofa |
| 5 | - |
- |
Gallus gallus |
| 5 | - |
- |
Sus scrofa |
| 5.3 | - |
assay at | Gallus gallus |
| 5.3 | - |
assay at | Sus scrofa |
| 5.3 | - |
assay at | Equus caballus |
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