Inhibitors | Comment | Organism | Structure |
---|---|---|---|
7,8-dihydroneopterin 3'-phosphate | 0.074 mM, 21% inhibition; inhibits 21% at a concentration of 0.074 mM, when the substrate is present at 0.032 mM | Escherichia coli | |
diphosphate | 1 mM, 89% inhibition | Escherichia coli | |
dTTP | 1 mM, 72% inhibition | Escherichia coli | |
additional information | no inhibition by dihydrofolic acid, dihydropteroic acid, dihydroneopterin and 6-hydroxy-methyl-dihydropterin | Escherichia coli | |
TTP | 1 mM, 72% inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
7,8-dihydroneopterin 3'-triphosphate | pH 8.5, 42°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | divalent cations are rerquired for the enzyme to function. Maximal activation is achieved with 8 mM Mg2+. Zn2+ and Ca2+ (both at 8 mM) are 2.4% and 7.1%, respectively, as effective as Mg2+. Mn2+ can not be tested because 7,8-dihydroneopterin triphosphate is rapidly and nonenzymatically decomposed in the presence of Mn2+ | Escherichia coli | |
Mg2+ | maximal activation is achieved with 8 nM Mg2+. Zn2+ and Ca2+ (both at 8 mM) are 2.4% and 7.1%, respectively, as effective as Mg2 | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17000 | - |
- |
Escherichia coli |
17000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydroneopterin 3'-triphosphate + H2O | Escherichia coli | the enzyme participates in a folate biosynthesis pathway | 7,8-dihydroneopterin 3'-phosphate + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | P0AFC0 | - |
- |
Purification (Comment) | Organism |
---|---|
partial | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydroneopterin 3'-triphosphate + H2O | - |
Escherichia coli | 7,8-dihydroneopterin 3'-phosphate + diphosphate | - |
? | |
7,8-dihydroneopterin 3'-triphosphate + H2O | the enzyme participates in a folate biosynthesis pathway | Escherichia coli | 7,8-dihydroneopterin 3'-phosphate + diphosphate | - |
? | |
additional information | no activity with ATP, GTP, dihydroneopterin phosphate, 4-nitrophenyl hosphate or diphosphate | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dihydroneopterin triphosphate pyrophosphohydrolase | - |
Escherichia coli |
H2-neopterin-PPP pyrophosphohydrolase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
- |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | - |
1 min, activity is destroyed | Escherichia coli |
100 | - |
1 min, enzyme is destroyed | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.1 | 9.7 | half-maximal activity at pH.1 and at pH 9.7 | Escherichia coli |
7.1 | 9.7 | half-maximal activity at pH 7.1 and at pH 9.7 | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme participates in a folate biosynthesis pathway | Escherichia coli |