Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanocaldococcus jannaschii |
Crystallization (Comment) | Organism |
---|---|
crystals are grown by hanging-drop vapor diffusion method, the crystal structure of the protein is determined at 2.2 A resolution | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
XTP | pH 7.6, 80°C | Methanocaldococcus jannaschii | |
0.15 | - |
ITP | pH 7.6, 80°C | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | NTPase activities are most efficient in the presence of either Mg2+ or Mn2+ at 80°C | Methanocaldococcus jannaschii | |
Mn2+ | NTPase activities are most efficient in the presence of either Mg2+ or Mn2+ at 80°C | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q57679 | - |
- |
Methanocaldococcus jannaschii DSM 2661 | Q57679 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ITP + H2O | catalytic efficiency (kcat/Km) is about 60% compared to the catalytic efficiency of XTP | Methanocaldococcus jannaschii | IMP + diphosphate | - |
? | |
ITP + H2O | catalytic efficiency (kcat/Km) is about 60% compared to the catalytic efficiency of XTP | Methanocaldococcus jannaschii DSM 2661 | IMP + diphosphate | - |
? | |
additional information | catalytic efficiency (kcat/Km) for GTP, dGTP, ATP, CTP or TTP is less than 1% compared to the catalytic efficiency of XTP | Methanocaldococcus jannaschii | ? | - |
? | |
additional information | catalytic efficiency (kcat/Km) for GTP, dGTP, ATP, CTP or TTP is less than 1% compared to the catalytic efficiency of XTP | Methanocaldococcus jannaschii DSM 2661 | ? | - |
? | |
XTP + H2O | - |
Methanocaldococcus jannaschii | XMP + diphosphate | - |
? | |
XTP + H2O | - |
Methanocaldococcus jannaschii DSM 2661 | XMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
Mj0226 | locus name | Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
assay at | Methanocaldococcus jannaschii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
911.7 | - |
ITP | pH 7.6, 80°C | Methanocaldococcus jannaschii | |
1009 | - |
XTP | pH 7.6, 80°C | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Methanocaldococcus jannaschii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5998 | - |
ITP | pH 7.6, 80°C | Methanocaldococcus jannaschii | |
10200 | - |
XTP | pH 7.6, 80°C | Methanocaldococcus jannaschii |