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Literature summary for 3.6.1.64 extracted from

  • Tresaugues, L.; Lundbaeck, T.; Welin, M.; Flodin, S.; Nyman, T.; Silvander, C.; Graeslund, S.; Nordlund, P.
    Structural basis for the specificity of human NUDT16 and its regulation by inosine monophosphate (2015), PLoS ONE, 10, e0131507.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.64

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant A22V,structure forms an alpha/beta/alpha sandwich which is constituted by two beta-sheets, one being composed of two parallel beta-strands lined by two anti-parallel beta-strands and the second one by three anti-parallel beta-strands. The protein is decorated with five additional motifs being part of the hydrophobic core or serving roles to stabilize the dimer Homo sapiens

Protein Variants

Protein Variants Comment Organism
A22V crystallization data Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Homo sapiens
GDP
-
 Homo sapiens
IDP
-
 Homo sapiens
IMP product inhibition Homo sapiens
ITP
-
 Homo sapiens
XDP
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0062
-
 XDP pH 7.5, 37°C Homo sapiens
0.0082
-
 ITP pH 7.5, 37°C Homo sapiens
0.285
-
 ADP pH 7.5, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q96DE0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + H2O
-
 Homo sapiens AMP + phosphate
-
 ?
ITP + H2O
-
 Homo sapiens IDP + phosphate
-
 ?
additional information rate of the catalytic cycle is primarily regulated by the product-release step Homo sapiens ?
-
 ?
XDP + H2O
-
 Homo sapiens XMP + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer crystallization data Homo sapiens

Synonyms

Synonyms Comment Organism
U8 snoRNA-decapping enzyme
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.006
-
 XDP pH 7.5, 37°C Homo sapiens
0.013
-
 ITP pH 7.5, 37°C Homo sapiens
0.096
-
 ADP pH 7.5, 37°C Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0098
-
 IMP substrate IDP, pH 7.5, 37°C Homo sapiens
0.0163
-
 IMP substrate IDP, pH 7.5, 37°C Homo sapiens
0.0213
-
 IMP substrate GDP, pH 7.5, 37°C Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.024
-
 substrate IDP, pH 7.5, 37°C Homo sapiens IMP
0.795
-
 substrate GDP, pH 7.5, 37°C Homo sapiens IMP
3.174
-
 substrate IDP, pH 7.5, 37°C Homo sapiens IMP

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.52
-
 ADP pH 7.5, 37°C Homo sapiens
0.99
-
 XDP pH 7.5, 37°C Homo sapiens
1.55
-
 ITP pH 7.5, 37°C Homo sapiens