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Literature summary for 3.6.1.63 extracted from
- Intermediates in the transformation of phosphonates to phosphate by bacteria (2011), Nature, 480, 570-573.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Escherichia coli |  |
| Zn2+ | - |
Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified using gel filtration with a High Load Superdex 200 26/60 prep grade column followed by anion exchange chromatography | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| alpha-D-ribose 1-methylphosphonate 5-triphosphate + H2O = alpha-D-ribose 1-methylphosphonate 5-phosphate + diphosphate | when the hydrolysis reaction of D-ribose-5-triphosphate catalyzed by PhnM is conducted in oxygen-18 labeled water, the oxygen-18 is found exclusively in D-ribose-5-phosphate and not in diphosphate. Therefore, water attacks the alpha-phosphoryl group of D-ribose-5-triphosphate rather than the beta-phosphoryl group | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-ribose 1-methylphosphonate 5-triphosphate + H2O | - |
Escherichia coli | alpha-D-ribose 1-methylphosphonate 5-phosphate + diphosphate | - |
? | |
| D-ribose 5-diphosphate + H2O | - |
Escherichia coli | D-ribose 5-phosphate + phosphate | - |
? | |
| D-ribose 5-triphosphate + H2O | - |
Escherichia coli | D-ribose 5-phosphate + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phnM | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Escherichia coli |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 110 | - |
alpha-D-ribose 1-methylphosphonate 5-triphosphate | pH 8.5, temperature not specified in the publication | Escherichia coli | |
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Release 2023.1 (January 2023)
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