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Literature summary for 3.6.1.62 extracted from

  • Parrish, S.; Moss, B.
    Characterization of a second vaccinia virus mRNA-decapping enzyme conserved in poxviruses (2007), J. Virol., 81, 12973-12978.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Vaccinia virus

Protein Variants

Protein Variants Comment Organism
additional information decapping actiovity is abolished by point mutations in the Nudix hydrolase motif Vaccinia virus

Inhibitors

Inhibitors Comment Organism Structure
m7G5'ppp5'G D9 differs from D10 in having lower sensitivity to inhibition by nucleotide cap analogs unattached to RNA Vaccinia virus
N7-methylguanosine 5'-diphosphate D9 differs from D10 in having lower sensitivity to inhibition by nucleotide cap analogs unattached to RNA Vaccinia virus
N7-methylguanosine 5'-triphosphate D9 differs from D10 in having lower sensitivity to inhibition by nucleotide cap analogs unattached to RNA Vaccinia virus
uncapped RNA D9 differs from D10 in having greater sensitivity to inhibition by uncapped RNA Vaccinia virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
m7G5'ppp5'-mRNA + H2O Vaccinia virus D9 is expressed early in infection and D10 late. It is suggested that the two proteins enhance mRNA turnover and manipulate gene expression in a complementary and overlapping manner m7GDP + 5'-phospho-mRNA
-
?

Organism

Organism UniProt Comment Textmining
Vaccinia virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Vaccinia virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
m7G5'ppp5'-mRNA + H2O D9 is expressed early in infection and D10 late. It is suggested that the two proteins enhance mRNA turnover and manipulate gene expression in a complementary and overlapping manner Vaccinia virus m7GDP + 5'-phospho-mRNA
-
?
m7G5'ppp5'-mRNA + H2O decapping is specific for a methylated cap attached to RNA. D9 differs from D10 in requiring a longer capped RNA substrate for optimal activity. Only trace amounts of the 12- and 24-nt RNA substrates are cleaved. Higher activity with 36-, 48-, or 309-nt RNA substrates Vaccinia virus m7GDP + 5'-phospho-mRNA
-
?

Synonyms

Synonyms Comment Organism
D9 protein
-
Vaccinia virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Vaccinia virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Vaccinia virus

General Information

General Information Comment Organism
physiological function D9 is expressed early in infection and D10 late. It is suggested that the two proteins enhance mRNA turnover and manipulate gene expression in a complementary and overlapping manner Vaccinia virus