Cloned (Comment) | Organism |
---|---|
quantitative real-time RT-PCR expression analysis, recombinant expression of HA-tagged wild-type and mutant enzymes in transgenic soybean roots via Agrobacterium rhizogenes-mediated hairy root transformation, expression of His-tagged wild-type and mutant enzymes in Escherichia coli | Glycine max |
Protein Variants | Comment | Organism |
---|---|---|
D209A | site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme | Glycine max |
E182A | site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme | Glycine max |
Q216A | site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme | Glycine max |
S214A | site-directed mutagenesis, the mutant enzyme shows reduced activity compared to the wild-type enzyme | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | complete inhibition at 1 M | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.309 | - |
ADP | pH and temperature not specified in the publication | Glycine max | |
0.424 | - |
ATP | pH and temperature not specified in the publication | Glycine max |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the most effective activating divalent cation | Glycine max | |
additional information | activating cations in descending effectivity order: Ca2+, Mg2+, Ni2+, Co2+ = Mn2+ = Cd2+, Zn2+ = Cu2+ for ATPase activity, and Ca2+, Mg2+, Ni2+ = Co2+, Mn2+ = Cu2+, Cd2+ = Zn2+ for ADPase activity | Glycine max |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Glycine max | - |
root hair | - |
Glycine max | - |
root nodule | - |
Glycine max | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | ADPase activity of GS52 is consistently more than 1.5fold higher than the ATPase activity | Glycine max | AMP + phosphate | - |
? | |
ATP + 2 H2O | ADPase activity of GS52 is consistently more than 1.5fold higher than the ATPase activity | Glycine max | AMP + 2 phosphate | - |
? | |
CTP + 2 H2O | - |
Glycine max | CMP + 2 phosphate | - |
? | |
GDP + H2O | - |
Glycine max | GMP + phosphate | - |
? | |
GTP + 2 H2O | - |
Glycine max | GMP + 2 phosphate | - |
? | |
ITP + 2 H2O | - |
Glycine max | IMP + 2 phosphate | - |
? | |
additional information | GS52 enzyme exhibits broad substrate specificity, but its activity on pyrimidine nucleotides and diphosphate nucleotides is significantly higher than on ATP due to low specificity for the adenine base within the substrate binding pocket of the enzyme. No hydrolytic activity with AMP | Glycine max | ? | - |
? | |
UDP + H2O | - |
Glycine max | UMP + phosphate | - |
? | |
UTP + 2 H2O | - |
Glycine max | UMP + 2 phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | GS52 apyrase structure modeling of apo-form and tertiary complex with the nonhydrolyzable ATP analogue AMPPNP and cofactor Ca2+, overview | Glycine max |
Synonyms | Comment | Organism |
---|---|---|
ecto-apyrase | - |
Glycine max |
GS52 | - |
Glycine max |
General Information | Comment | Organism |
---|---|---|
evolution | GS52 is a member of the NTPDase/apyrase family | Glycine max |
physiological function | important role for the Glycine max ecto-apyrase GS52 in rhizobial root hair infection and root nodule formation | Glycine max |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.28 | - |
ATP | pH and temperature not specified in the publication | Glycine max | |
3.02 | - |
ADP | pH and temperature not specified in the publication | Glycine max |