Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3), refolding of the enzyme from bacterial inclusion bodies | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C139S | mutant enzyme lacking the single free sulfhydryl is more stable than the wild-type enzyme under refolding conditions, 10-15°C, 2-3 days. The mutant enzyme is less susceptible to oxidative inactivation than the wild-type enzyme | Homo sapiens |
General Stability | Organism |
---|---|
mutant enzyme C139S lacking the single free sulfhydryl is more stable than the wild-type enzyme under refolding conditions, 10-15°C, 2-3 days | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
GDP | in presence of Ca2+, wild-type and mutant enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates, slightly more effective than Mg2+ | Homo sapiens | |
Mg2+ | activates, slightly less effective than Ca2+ | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O75354 | - |
- |
Oxidation Stability | Organism |
---|---|
the mutant enzyme C139S is less susceptible to oxidative inactivation than the wild-type enzyme | Homo sapiens |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | nevertheless the bacterially expressed refolded enzyme is not glycosylated, it has high specific activity, indicating that glycosylation is not essential for folding or nucleotidase activity of NTPDase6 | Homo sapiens |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Renatured (Comment) | Organism |
---|---|
refolding of the enzyme from bacterial inclusion bodies. For wild-type enzyme optimal refolding ocurs at 10-15°C over 2-3 days | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | 24.4% of the specific activity with GDP | Homo sapiens | AMP + phosphate | - |
? | |
CDP + H2O | 12.9% of the specific activity with GDP | Homo sapiens | CMP + phosphate | - |
? | |
CTP + H2O | 3.8% of the specific activity with GDP | Homo sapiens | CDP + phosphate | - |
? | |
GDP + H2O | best substrate | Homo sapiens | GMP + phosphate | - |
? | |
GTP + H2O | 4.4% of the specific activity with GDP | Homo sapiens | GDP + phosphate | - |
? | |
IDP + H2O | 55.5% of the specific activity with GDP | Homo sapiens | IMP + phosphate | - |
? | |
ITP + H2O | 6% of the specific activity with GDP | Homo sapiens | IDP + phosphate | - |
? | |
additional information | no activity with ATP | Homo sapiens | ? | - |
? | |
UDP + H2O | 19.8% of the specific activity with GDP | Homo sapiens | UMP + phosphate | - |
? | |
UTP + H2O | 1.2% of the specific activity with GDP | Homo sapiens | UDP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
NTPDase6 | - |
Homo sapiens |
NTPDase6 (CD39L2) nucleotidase | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.4 | - |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.7 | 7.8 | pH 6.7: about 50% of maximal activity with Mg2+ or Ca2+, about 80% of maximal activity with Ca2+, about 70% of maximal activity with Mg2+ | Homo sapiens |