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Literature summary for 3.6.1.59 extracted from
- Crystal structures of human DcpS in ligand-free and m7GDP-bound forms suggest a dynamic mechanism for scavenger mRNA decapping (2005), J. Mol. Biol., 347, 707-718.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging-drop vapor-diffusion method at 20°C. Structures of DcpS in ligand-free form and in a complex with m7GDP. Examination of the crystallographic B-factors indicates that the N-terminal domain in apo-DcpS is inherently flexible, and in a dynamic state ready for substrate binding and product release | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| m7G5'ppp5'N(3'ppp5'N)n + H2O | Homo sapiens | eukaryotic cells utilize DcpS, a scavenger decapping enzyme, to degrade the residual cap structure following 30-50 mRNA decay, thereby preventing the premature decapping of the capped long mRNA and misincorporation of methylated nucleotides in nucleic acids | 7-methylguanosine 5'-phosphate + pp5'N(3'ppp5'N)n | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q96C86 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] + H2O = N7-methylguanosine 5'-phosphate + a 5'-diphospho-[mRNA] | (1) | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7-methylguanosine 5'-diphosphate + H2O | - |
Homo sapiens | 7-methylguanosine 5'-phosphate + phosphate | - |
? | |
| m7G5'ppp5'N(3'ppp5'N)n + H2O | eukaryotic cells utilize DcpS, a scavenger decapping enzyme, to degrade the residual cap structure following 30-50 mRNA decay, thereby preventing the premature decapping of the capped long mRNA and misincorporation of methylated nucleotides in nucleic acids | Homo sapiens | 7-methylguanosine 5'-phosphate + pp5'N(3'ppp5'N)n | - |
? | |
| m7G5'ppp5'N(3'ppp5'N)n + H2O | Tyr273 seems to play an important role in cap binding and product release | Homo sapiens | 7-methylguanosine 5'-phosphate + pp5'N(3'ppp5'N)n | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | in contrast, and similar to the m7GpppG-DcpS complex, DcpS with bound m7GDP is an asymmetric dimer in which the closed state appears to be the substrate-bound complex, whereas the open state mimics the product-bound complex. apo-DcpS is a symmetric dimer | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Dcps | - |
Homo sapiens |
| m7GpppN m7GMP phosphohydrolase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | eukaryotic cells utilize DcpS, a scavenger decapping enzyme, to degrade the residual cap structure following 30-50 mRNA decay, thereby preventing the premature decapping of the capped long mRNA and misincorporation of methylated nucleotides in nucleic acids | Homo sapiens |
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