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Literature summary for 3.6.1.59 extracted from

  • Malys, N.; McCarthy, J.E.
    Dcs2, a novel stress-induced modulator of m7GpppX pyrophosphatase activity that locates to P bodies (2006), J. Mol. Biol., 363, 370-382.
    View publication on PubMed
Search for more literature for 3.6.1.59

Inhibitors

Inhibitors Comment Organism Structure
Dcs2 protein Dcs2 is recruited into cytoplasmic P bodies, its inhibitory function may be focused in these centres of mRNA storage/turnover. Dcs2 is a stress-induced regulatory protein that modulates m7GpppX pyrophosphatase activity Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00014
-
 m7G5'ppp5'G pH 7.0, 30°C, Dcs1 homodimer Saccharomyces cerevisiae
0.00026
-
 7-methylguanosine 5'-diphosphate pH 7.0, 30°C, Dcs1 homodimer Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7-methylguanosine 5'-diphosphate + H2O Saccharomyces cerevisiae cleavage of 7-methylguanosine 5'-diphosphate generated by Dcp1/Dcp2-mediated decapping in the 5' to 3' decay pathway 7-methylguanosine 5'-phosphate + phosphate
-
 ?
m7G5'ppp5'N(3'ppp5'N)n + H2O Saccharomyces cerevisiae cleavage of 5' end m7G-oligoribonucleotide fragments generated by 3' to 5' exonucleolytic decay 7-methylguanosine 5'-phosphate + pp5'N(3'ppp5'N)n
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q06151
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-methylguanosine 5'-diphosphate + H2O
-
 Saccharomyces cerevisiae 7-methylguanosine 5'-phosphate + phosphate
-
 ?
7-methylguanosine 5'-diphosphate + H2O cleavage of 7-methylguanosine 5'-diphosphate generated by Dcp1/Dcp2-mediated decapping in the 5' to 3' decay pathway Saccharomyces cerevisiae 7-methylguanosine 5'-phosphate + phosphate
-
 ?
m7G5'ppp5'G + H2O
-
 Saccharomyces cerevisiae 7-methylguanosine 5'-phosphate + ?
-
 ?
m7G5'ppp5'N(3'ppp5'N)n + H2O cleavage of 5' end m7G-oligoribonucleotide fragments generated by 3' to 5' exonucleolytic decay Saccharomyces cerevisiae 7-methylguanosine 5'-phosphate + pp5'N(3'ppp5'N)n
-
 ?
m7G5'ppp5'N(3'ppp5'N)n + H2O n = 1-8 Saccharomyces cerevisiae 7-methylguanosine 5'-phosphate + pp5'N(3'ppp5'N)n
-
 ?

Subunits

Subunits Comment Organism
dimer Dcs1 is active as a homodimer. Dcs2 protein forms a heterodimer together with Dcs1, both modulating Dcs1 substrate specificity and suppressing its turnover number Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
Dcps
-
 Saccharomyces cerevisiae
Dcs1
-
 Saccharomyces cerevisiae
m7GpppX pyrophosphatase
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0017
-
 7-methylguanosine 5'-diphosphate pH 7.0, 30°C, Dcs1 homodimer Saccharomyces cerevisiae
0.012
-
 m7G5'ppp5'G pH 7.0, 30°C, Dcs1 homodimer Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction depletion of Dcs1, reduces chronological life span, possibly by modulating m7G misincorporation into nucleic acids. This can potentially link control of mRNA metabolism with senescence Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.5
-
 7-methylguanosine 5'-diphosphate pH 7.0, 30°C, Dcs1 homodimer Saccharomyces cerevisiae
86
-
 m7G5'ppp5'G pH 7.0, 30°C, Dcs1 homodimer Saccharomyces cerevisiae