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Literature summary for 3.6.1.58 extracted from

  • Ishibashi, T.; Hayakawa, H.; Sekiguchi, M.
    A novel mechanism for preventing mutations caused by oxidation of guanine nucleotides (2003), EMBO Rep., 4, 479-483.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.58

Cloned(Commentary)

Cloned (Comment) Organism
when NUDT5 is expressed in Escherichia coli mutT-cells as a His-tagged protein, the increased frequency of spontaneous mutations is decreased to normal levels Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.77
-
 8-oxo-dGDP pH 8.0, 30°C Homo sapiens
7.1
-
 dGDP pH 8.0, 30°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
8-oxo-dGDP + H2O Homo sapiens NUDT5 might have a much greater role than MTH1 in preventing the occurrence of mutations that are caused by the misincorporation of 8-oxoguanine in human cells. NUDT5 has another role in promoting the MTH1 reaction, in removing its inhibitor, 8-oxo-dGDP 8-oxo-dGMP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UKK9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8-oxo-dGDP + H2O NUDT5 might have a much greater role than MTH1 in preventing the occurrence of mutations that are caused by the misincorporation of 8-oxoguanine in human cells. NUDT5 has another role in promoting the MTH1 reaction, in removing its inhibitor, 8-oxo-dGDP Homo sapiens 8-oxo-dGMP + phosphate
-
 ?
8-oxo-dGDP + H2O 8-oxo-dGDP is a specific substrate for the NUDT5 protein Homo sapiens 8-oxo-dGMP + phosphate
-
 ?
dGDP + H2O poor substrate. Vmax/Km is 32% of value for 8-oxo-dGDP Homo sapiens dGMP + phosphate
-
 ?
additional information NUDT5 hydrolyses 8-oxo-dGTP only at very low levels (Vmax/Km is 0.07% of the value for 8-oxo-dGDP) Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
NUDT
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens

General Information

General Information Comment Organism
physiological function NUDT5 might have a much greater role than MTH1 in preventing the occurrence of mutations that are caused by the misincorporation of 8-oxoguanine in human cells Homo sapiens