Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.6.1.58 extracted from

  • Kamiya, H.; Hori, M.; Arimori, T.; Sekiguchi, M.; Yamagata, Y.; Harashima, H.
    NUDT5 hydrolyzes oxidized deoxyribonucleoside diphosphates with broad substrate specificity (2009), DNA Repair, 8, 1250-1254.
    View publication on PubMed
Search for more literature for 3.6.1.58

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
 8-oxo-dGDP pH 8.0, 30°C Homo sapiens
0.0029
-
 8-oxo-dADP pH 8.0, 30°C Homo sapiens
0.004
-
 5-formyl-dUDP pH 8.0, 30°C Homo sapiens
0.0076
-
 dGDP pH 8.0, 30°C Homo sapiens
0.0088
-
 2-hydroxy-dADP pH 8.0, 30°C Homo sapiens
0.0126
-
 dADP pH 8.0, 30°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UKK9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-dADP + H2O kcat/KM is 15% of the wild-type value Homo sapiens 2-hydroxy-dAMP + phosphate
-
 ?
5-formyl-dUDP + H2O kcat/KM is 30% of the wild-type value Homo sapiens 5-formyl-dUMP + phosphate
-
 ?
8-oxo-dADP + H2O 8-oxo-dADP is hydrolyzed slightly more efficiently than 8-oxo-dGDP Homo sapiens 8-oxo-dAMP + phosphate
-
 ?
8-oxo-dGDP + H2O NUDT5 protein is a hydrolyzing enzyme with broad substrate specificity for oxidized deoxyribonucleoside diphosphates Homo sapiens 8-oxo-dGMP + phosphate
-
 ?
dADP + H2O kcat/KM is 16% of the wild-type value Homo sapiens dAMP + phosphate
-
 ?
dGDP + H2O
-
 Homo sapiens dGMP + phosphate
-
 ?
additional information the hydrolyses of 2-hydroxy-dADP and 5-formyl-dUDP are less effective, with 0.4- and 0.5-fold efficiencies relative to that of 8-oxo-dGDP, respectively. The hydrolyses of the triphosphate derivatives are less efficient than those of the diphosphate counterparts Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
NUDT5
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0035
-
 5-formyl-dUDP pH 8.0, 30°C Homo sapiens
0.004
-
 2-hydroxy-dADP pH 8.0, 30°C Homo sapiens
0.006
-
 dADP pH 8.0, 30°C Homo sapiens
0.006
-
 8-oxo-dGDP pH 8.0, 30°C Homo sapiens
0.009
-
 8-oxo-dADP pH 8.0, 30°C Homo sapiens
0.015
-
 dGDP pH 8.0, 30°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.45
-
 2-hydroxy-dADP pH 8.0, 30°C Homo sapiens
0.48
-
 dADP pH 8.0, 30°C Homo sapiens
0.88
-
 5-formyl-dUDP pH 8.0, 30°C Homo sapiens
2.9
-
 8-oxo-dGDP pH 8.0, 30°C Homo sapiens
3.1
-
 8-oxo-dADP pH 8.0, 30°C Homo sapiens