Cloned (Comment) | Organism |
---|---|
the increase in the production of erroneous proteins by oxidative damage is 28fold over the wild-type cells in Escherichia coli mutT deficient cells. By the expression of MTH1 in the cells, it is reduced to 1.2fold | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.044 | - |
8-oxo-GDP | pH 8.0, 30°C | Homo sapiens | |
0.29 | - |
8-oxo-GTP | pH 8.0, 30°C | Homo sapiens | |
0.79 | - |
GTP | pH 8.0, 30°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
8-oxo-GTP + H2O | Homo sapiens | this enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP | 8-oxo-GMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8-oxo-GDP + H2O | Vmax/Km is 4% of the value for 8-oxo-GTP | Homo sapiens | 8-oxo-GMP + phosphate | - |
? | |
8-oxo-GTP + H2O | this enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP | Homo sapiens | 8-oxo-GMP + diphosphate | - |
? | |
8-oxo-GTP + H2O | the MTH1 protein has the ability to cleave the phosphoanhydride bond between the alpha and the beta phosphate of 8-oxoguanine-containing nucleoside di- and triphosphates. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP | Homo sapiens | 8-oxo-GMP + diphosphate | - |
? | |
GTP + H2O | Vmax/Km is 31% of the value for 8-oxo-GTP | Homo sapiens | GMP + diphosphate | - |
? | |
additional information | Vmax/Km for GDP is 0.3% of the value for 8-oxo-GTP | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MTH1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | is produced in cells by reactive oxygen species normally formed during cellular metabolic processes. This oxidized base can pair with both adenine and cytosine, and thus the existence of this base in messenger RNA would cause translational errors. The elimination of 8-oxoguanine-containing ribonucleotides from the precursor pool is important to ensure accurate protein synthesis. Both NUDT5 and MTH1 are involved in this process in human cells | Homo sapiens |