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Literature summary for 3.6.1.56 extracted from

  • Ishibashi, T.; Hayakawa, H.; Ito, R.; Miyazawa, M.; Yamagata, Y.; Sekiguchi, M.
    Mammalian enzymes for preventing transcriptional errors caused by oxidative damage (2005), Nucleic Acids Res., 33, 3779-3784.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
the increase in the production of erroneous proteins by oxidative damage is 28fold over the wild-type cells in Escherichia coli mutT deficient cells. By the expression of MTH1 in the cells, it is reduced to 1.2fold Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.044
-
8-oxo-GDP pH 8.0, 30°C Homo sapiens
0.29
-
8-oxo-GTP pH 8.0, 30°C Homo sapiens
0.79
-
GTP pH 8.0, 30°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
8-oxo-GTP + H2O Homo sapiens this enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP 8-oxo-GMP + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8-oxo-GDP + H2O Vmax/Km is 4% of the value for 8-oxo-GTP Homo sapiens 8-oxo-GMP + phosphate
-
?
8-oxo-GTP + H2O this enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP Homo sapiens 8-oxo-GMP + diphosphate
-
?
8-oxo-GTP + H2O the MTH1 protein has the ability to cleave the phosphoanhydride bond between the alpha and the beta phosphate of 8-oxoguanine-containing nucleoside di- and triphosphates. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP Homo sapiens 8-oxo-GMP + diphosphate
-
?
GTP + H2O Vmax/Km is 31% of the value for 8-oxo-GTP Homo sapiens GMP + diphosphate
-
?
additional information Vmax/Km for GDP is 0.3% of the value for 8-oxo-GTP Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
MTH1
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens

General Information

General Information Comment Organism
physiological function is produced in cells by reactive oxygen species normally formed during cellular metabolic processes. This oxidized base can pair with both adenine and cytosine, and thus the existence of this base in messenger RNA would cause translational errors. The elimination of 8-oxoguanine-containing ribonucleotides from the precursor pool is important to ensure accurate protein synthesis. Both NUDT5 and MTH1 are involved in this process in human cells Homo sapiens