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Literature summary for 3.6.1.56 extracted from

  • Mishima, M.; Sakai, Y.; Itoh, N.; Kamiya, H.; Furuichi, M.; Takahashi, M.; Yamagata, Y.; Iwai, S.; Nakabeppu, Y.; Shirakawa, M.
    Structure of human MTH1, a Nudix family hydrolase that selectively degrades oxidized purine nucleoside triphosphates (2004), J. Biol. Chem., 279, 33806-33815.
    View publication on PubMed
Search for more literature for 3.6.1.56

Protein Variants

Protein Variants Comment Organism
D119A the mutant enzyme has about half of the wild-type activity for 8-oxo-dGTP but shows almost no activity for 2-hydroxy-dATP Homo sapiens
D119N the mutant enzyme has about half of the wild-type activity for 8-oxo-dGTP but shows almost no activity for 2-hydroxy-dATP Homo sapiens
N33A mutation decreases catalytic activity toward 2-hydroxy-dATP to 5% of the wild-type activity. The mutant shows 14% of the wild-type 8-oxo-dGTP activity Homo sapiens
N33E mutation decreases catalytic activity toward 2-hydroxy-dATP to 53% of the wild-type activity. Activity towards 8-oxo-dGTP is totally abolished Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-hydroxy-dATP + H2O Homo sapiens human MTH1 hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP and 2-oxo-dATP, to monophosphates, thereby preventing the misincorporation of these oxidized nucleotides during replication 2-hydroxy-dAMP + diphosphate
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 ?
8-oxo-dGTP + H2O Homo sapiens human MTH1 hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP and 2-oxo-dATP, to monophosphates, thereby preventing the misincorporation of these oxidized nucleotides during replication. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP 8-oxo-dGMP + diphosphate
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P36639
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-dATP + H2O
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 Homo sapiens 2-hydroxy-dAMP + diphosphate
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 ?
2-hydroxy-dATP + H2O human MTH1 hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP and 2-oxo-dATP, to monophosphates, thereby preventing the misincorporation of these oxidized nucleotides during replication Homo sapiens 2-hydroxy-dAMP + diphosphate
-
 ?
8-oxo-dGTP + H2O human MTH1 hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP and 2-oxo-dATP, to monophosphates, thereby preventing the misincorporation of these oxidized nucleotides during replication. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP Homo sapiens 8-oxo-dGMP + diphosphate
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 ?
8-oxo-dGTP + H2O MTH1 catalyzes hydrolysis of 8-oxo-dGTP through nucleophilic substitution of water at the beta-phosphate. The side chains of Asp119 and Asn33 play an essential role in 2-OH-dATP recognition, whereas the indole ring of Trp117 may be important for interacting with the purine bases of substrate nucleotides. Solution structure of MTH1 solved by multidimensional heteronuclear NMR spectroscopy, identification of the substrate interaction pocket. This enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP Homo sapiens 8-oxo-dGMP + diphosphate
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 ?

Synonyms

Synonyms Comment Organism
MTH1
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 Homo sapiens