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Literature summary for 3.6.1.56 extracted from

  • Fujikawa, K.; Kamiya, H.; Yakushiji, H.; Fujii, Y.; Nakabeppu, Y.; Kasai, H.
    The oxidized forms of dATP are substrates for the human MutT homologue, the hMTH1 protein (1999), J. Biol. Chem., 274, 18201-18205.
    View publication on PubMed
Search for more literature for 3.6.1.56

Inhibitors

Inhibitors Comment Organism Structure
2'-deoxy-8-hydroxyguanosine 5'-triphosphate
-
 Homo sapiens
2-hydroxy-dADP competitively inhibits both the 2-hydroxy-dATP hydrolase and 8-hydroxy-dGTP hydrolase activities of hMTH1 Homo sapiens
8-oxo-dADP
-
 Homo sapiens
8-oxo-dGDP competitively inhibits both the 2-hydroxy-dATP hydrolase and 8-hydroxy-dGTP hydrolase activities of hMTH1 Homo sapiens
8-oxo-dGTP
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0083
-
 2-hydroxy-dATP pH 8.0, 30°C Homo sapiens
0.0139
-
 8-oxo-dATP pH 8.0, 30°C Homo sapiens
0.0152
-
 8-oxo-dGTP pH 8.0, 30°C Homo sapiens
5.7
-
 2-hydroxy-dATP pH 7.2, 30°C Homo sapiens
12.8
-
 8-oxo-dGTP pH 7.2, 30°C Homo sapiens
258
-
 dGTP pH 8.0, 30°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-hydroxy-dATP + H2O Homo sapiens
-
 2-hydroxy-dAMP + diphosphate
-
 ?
8-oxo-dGTP + H2O Homo sapiens this enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP 8-oxo-dGMP + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-dATP + H2O
-
 Homo sapiens 2-hydroxy-dAMP + diphosphate
-
 ?
2-hydroxy-dATP + H2O hydrolyzed more efficiently and with higher affinity than 8-oxo-dATP. Preferential hydrolysis of 2-oxo-dATP over 8-oxo-dGTP is observed at all of the pH values tested (pH 7.2 to pH 8.8). A 5fold difference in the hydrolysis efficiencies for 2-oxo-dATP over 8-oxo-dGTP is found at pH 7.2. In addition to the normal form of hMTH1 (valine 83), the variant form of the protein (methionine 83) also hydrolyzes 2-oxo-dATP more efficiently than 8-oxo-dGTP Homo sapiens 2-hydroxy-dAMP + diphosphate
-
 ?
8-oxo-dATP + H2O hydrolyzed as efficiently as 8-oxo-dGTP Homo sapiens 8-oxo-dAMP + diphosphate
-
 ?
8-oxo-dGTP + H2O this enzyme is a 2-hydroxy-dATP diphosphatase that also exhibits activity with 8-oxo-dGTP Homo sapiens 8-oxo-dGMP + diphosphate
-
 ?
dGTP + H2O
-
 Homo sapiens dGMP + diphosphate
-
 ?
additional information no hydrolysis of (R)-8,5'-cyclodATP, 5-oxo-dCTP, and 5-formyl-dUTP Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
hMTH1
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1
-
 8-oxo-dGTP pH 7.2, 30°C Homo sapiens
4.7
-
 2-hydroxy-dATP pH 7.2, 30°C Homo sapiens
10.8
-
 8-oxo-dATP pH 8.0, 30°C Homo sapiens
12.3
-
 8-oxo-dGTP pH 8.0, 30°C Homo sapiens
13.9
-
 2-hydroxy-dATP pH 8.0, 30°C Homo sapiens
15.7
-
 dGTP pH 8.0, 30°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7 8.8 hydrolysis of 2-hydroxy-dATP Homo sapiens
7.7 8.2 hydrolysis of 8-oxo-dGTP Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7.2 8.8 pH 7.2: about 60% of maximal activity, pH 8.8: about 95% of maximal activity, hydrolysis of 2-hydroxy-dATP Homo sapiens
7.7 8.8 pH 7.2: about 15% of maximal activity, pH 7.7: about 95% of maximal activity, pH 8.8: about 50% of maximal activity, hydrolysis of 8-oxo-dGTP Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0002
-
 8-oxo-dADP pH 8.0, 30°C, inhibition of 8-oxo-dGTPase activity Homo sapiens
0.00022
-
 8-oxo-dADP pH 8.0, 30°C, inhibition of 2-hydroxy-dATPase activity Homo sapiens
0.00051
-
 8-oxo-dGDP pH 8.0, 30°C, inhibition of 8-oxo-dGTPase activity Homo sapiens
0.00072
-
 8-oxo-dGDP pH 8.0, 30°C, inhibition of 2-hydroxy-dATPase activity Homo sapiens
0.0097
-
 2'-deoxy-8-hydroxyguanosine 5'-triphosphate pH 8.0, 30°C, inhibition of 2-hydroxy-dATPase activity Homo sapiens
0.0129
-
 8-oxo-dGTP pH 8.0, 30°C, inhibition of 2-hydroxy-dATPase activity Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6
-
 dGTP pH 8.0, 30°C Homo sapiens
16
-
 8-oxo-dGTP pH 7.2, 30°C Homo sapiens
78
-
 8-oxo-dATP pH 8.0, 30°C Homo sapiens
81
-
 8-oxo-dGTP pH 8.0, 30°C Homo sapiens
83
-
 2-hydroxy-dATP pH 7.2, 30°C Homo sapiens
168
-
 2-hydroxy-dATP pH 8.0, 30°C Homo sapiens