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Literature summary for 3.6.1.55 extracted from
- Structure of a Nudix hydrolase (MutT) in the Mg2+-bound state from Bartonella henselae, the bacterium responsible for cat scratch fever (2011), Acta Crystallogr. Sect. F, 67, 1078-1083.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3)-R3-pRARE2 cells | Bartonella henselae |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting drop vapor diffusion method, using 0.1 M HEPES, pH 7.5, 10% (w/v) PEG 4000, 0.1 M MgCl2 | Bartonella henselae |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | the enzyme binds catalytically essential Mg2+ | Bartonella henselae |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bartonella henselae | A0A0H3LW43 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel Sepharose column chromatography and Superdex 75 gel filtration | Bartonella henselae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydro-8-oxo-GTP + H2O | - |
Bartonella henselae | 7,8-dihydro-8-oxo-GMP + diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MutT | - |
Bartonella henselae |
| Nudix hydrolase | - |
Bartonella henselae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
the enzyme irreversibly unfolds and precipitates out of solution upon heating, with a melting temperature of 60°C | Bartonella henselae |
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