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Literature summary for 3.6.1.54 extracted from

  • Metzger, L.E.; Raetz, C.R.
    An alternative route for UDP-diacylglucosamine hydrolysis in bacterial lipid A biosynthesis (2010), Biochemistry, 49, 6715-6726.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in lpxh-deficient Escherichia coli CcI 21b cells Caulobacter vibrioides

Inhibitors

Inhibitors Comment Organism Structure
EDTA complete inhibition at 2 or 0.2 mM Caulobacter vibrioides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.105
-
UDP-2,3-diacylglucosamine in 100 mM sodium acetate, 50 mM bis(2-hydroxyethyl)imino-tris(hydroxymethyl)hexane, and 50 mM Tris, 2 mM MgCl2, at 30°C, pH not specified in the publication Caulobacter vibrioides

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Caulobacter vibrioides 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ the apparent specific activity of purified enzyme increases 6fold in the presence of added Co2+ Caulobacter vibrioides
Mg2+ the apparent specific activity of purified enzyme increases 10fold in the presence of Mg2+ versus no added metal. Enzyme activity is maximal at 0.2 mM Mg2+ and remains approximately the same up to 20 mM Mg2+ Caulobacter vibrioides
Mn2+ the apparent specific activity of purified enzyme increases 6fold in the presence of added Mn2+ Caulobacter vibrioides

Organic Solvent Stability

Organic Solvent Comment Organism
Triton X-100 while stimulated about 3fold in the presence of 0.05% (w/v) Triton X-100, the apparent activity does not decrease at high concentrations of Triton X-100 Caulobacter vibrioides

Organism

Organism UniProt Comment Textmining
Caulobacter vibrioides A0A0H3C8Q1
-
-
Caulobacter vibrioides CB15 A0A0H3C8Q1
-
-

Purification (Commentary)

Purification (Comment) Organism
High-Trap Q Sepharose column chromatography and Superdex 200 gel filtration Caulobacter vibrioides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25
-
membane-free lysate, in 100 mM sodium acetate, 50 mM bis(2-hydroxyethyl)imino-tris(hydroxymethyl)hexane, and 50 mM Tris, 2 mM MgCl2, at 30°C, pH not specified in the publication Caulobacter vibrioides
30
-
after 1.2fold purification, in 100 mM sodium acetate, 50 mM bis(2-hydroxyethyl)imino-tris(hydroxymethyl)hexane, and 50 mM Tris, 2 mM MgCl2, at 30°C, pH not specified in the publication Caulobacter vibrioides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-2,3-diacylglucosamine + H2O the enzyme is selective for UDP-2,3-diacylglucosamine Caulobacter vibrioides 2,3-diacylglucosamine 1-phosphate + UMP
-
?
UDP-2,3-diacylglucosamine + H2O the enzyme is selective for UDP-2,3-diacylglucosamine Caulobacter vibrioides CB15 2,3-diacylglucosamine 1-phosphate + UMP
-
?

Synonyms

Synonyms Comment Organism
LpxI
-
Caulobacter vibrioides
UDP-2,3-diacylglucosamine pyrophosphatase
-
Caulobacter vibrioides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9
-
Caulobacter vibrioides

General Information

General Information Comment Organism
malfunction UDP-2,3-diacylglucosamine-deficiency results in lethality Caulobacter vibrioides