Cloned (Comment) | Organism |
---|---|
expressed in lpxh-deficient Escherichia coli CcI 21b cells | Caulobacter vibrioides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | complete inhibition at 2 or 0.2 mM | Caulobacter vibrioides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.105 | - |
UDP-2,3-diacylglucosamine | in 100 mM sodium acetate, 50 mM bis(2-hydroxyethyl)imino-tris(hydroxymethyl)hexane, and 50 mM Tris, 2 mM MgCl2, at 30°C, pH not specified in the publication | Caulobacter vibrioides |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Caulobacter vibrioides | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | the apparent specific activity of purified enzyme increases 6fold in the presence of added Co2+ | Caulobacter vibrioides | |
Mg2+ | the apparent specific activity of purified enzyme increases 10fold in the presence of Mg2+ versus no added metal. Enzyme activity is maximal at 0.2 mM Mg2+ and remains approximately the same up to 20 mM Mg2+ | Caulobacter vibrioides | |
Mn2+ | the apparent specific activity of purified enzyme increases 6fold in the presence of added Mn2+ | Caulobacter vibrioides |
Organic Solvent | Comment | Organism |
---|---|---|
Triton X-100 | while stimulated about 3fold in the presence of 0.05% (w/v) Triton X-100, the apparent activity does not decrease at high concentrations of Triton X-100 | Caulobacter vibrioides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caulobacter vibrioides | A0A0H3C8Q1 | - |
- |
Caulobacter vibrioides CB15 | A0A0H3C8Q1 | - |
- |
Purification (Comment) | Organism |
---|---|
High-Trap Q Sepharose column chromatography and Superdex 200 gel filtration | Caulobacter vibrioides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25 | - |
membane-free lysate, in 100 mM sodium acetate, 50 mM bis(2-hydroxyethyl)imino-tris(hydroxymethyl)hexane, and 50 mM Tris, 2 mM MgCl2, at 30°C, pH not specified in the publication | Caulobacter vibrioides |
30 | - |
after 1.2fold purification, in 100 mM sodium acetate, 50 mM bis(2-hydroxyethyl)imino-tris(hydroxymethyl)hexane, and 50 mM Tris, 2 mM MgCl2, at 30°C, pH not specified in the publication | Caulobacter vibrioides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-2,3-diacylglucosamine + H2O | the enzyme is selective for UDP-2,3-diacylglucosamine | Caulobacter vibrioides | 2,3-diacylglucosamine 1-phosphate + UMP | - |
? | |
UDP-2,3-diacylglucosamine + H2O | the enzyme is selective for UDP-2,3-diacylglucosamine | Caulobacter vibrioides CB15 | 2,3-diacylglucosamine 1-phosphate + UMP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LpxI | - |
Caulobacter vibrioides |
UDP-2,3-diacylglucosamine pyrophosphatase | - |
Caulobacter vibrioides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Caulobacter vibrioides |
General Information | Comment | Organism |
---|---|---|
malfunction | UDP-2,3-diacylglucosamine-deficiency results in lethality | Caulobacter vibrioides |