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Literature summary for 3.6.1.45 extracted from
- Characterization of mouse UDP-glucose pyrophosphatase, a Nudix hydrolase encoded by the Nudt14 gene (2009), Biochem. Biophys. Res. Commun., 390, 1414-1418.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 (DE3) cells | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
ADP-D-ribose | in 50 mM Tris-HCl, pH 8.0, 10 mM Mg2+, at 37°C | Mus musculus |  |
| 4.1 | - |
UDP-D-glucose | in 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, at 37°C | Mus musculus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activity is dependent on the presence of Mg2+ | Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26700 | - |
2 * 26700, calculated from amino acid sequence | Mus musculus |
| 30000 | - |
2 * 30000, SDS-PAGE | Mus musculus |
| 54000 | - |
SDS-PAGE | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | Q9D142 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA resin column chromatography | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipose tissue | - |
Mus musculus | - |
| brain | - |
Mus musculus | - |
| heart | - |
Mus musculus | - |
| kidney | - |
Mus musculus | - |
| liver | - |
Mus musculus | - |
| lung | - |
Mus musculus | - |
| pancreas | - |
Mus musculus | - |
| skeletal muscle | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-D-ribose + H2O | based on Vmax/Km values, the enzyme is about 20fold more active toward ADP-D-ribose as compared to UDP-D-glucose | Mus musculus | AMP + alpha-D-ribose 1-phosphate | - |
? | |
| UDP-D-glucose + H2O | - |
Mus musculus | UMP + alpha-D-glucose 1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 30000, SDS-PAGE | Mus musculus |
| homodimer | 2 * 26700, calculated from amino acid sequence | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDP-glucose pyrophosphatase | - |
Mus musculus |
| UGPPase | - |
Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Mus musculus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9.5 | - |
Mus musculus |
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