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Literature summary for 3.6.1.45 extracted from

  • Alves-Pereira, I.; Canales, J.; Cabezas, A.; Cordero, P.M.; Costas, M.J.; Cameselle, J.C.
    CDP-alcohol hydrolase, a very efficient activity of the 5-nucleotidase/UDP-sugar hydrolase encoded by the ushA gene of Yersinia intermedia and Escherichia coli (2008), J. Bacteriol., 190, 6153-6161.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.45

Cloned(Commentary)

Cloned (Comment) Organism
gene ushA, DNA and amino acid sequence determination and analysis, expression analysis by RT-PCR Yersinia intermedia
gene ushA, overexpression of the enzyme Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information ushA knockouts are unable to grow on AMP as the only carbon source and are significantly delayed or impaired in their growth on other purine nucleotides. Growth on nonadenine purine nucleotides is made possible by an acid phosphatase encoded by the aphA gene, which at neutral pH acts as an efficient 5'-nucleotidase of purine nucleotides, except AMP. Double knockouts in ushA and aphA do not grow on nucleotides AMP Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of all the enzyme activites, overview Escherichia coli
additional information
-
 additional information kinetics of all the enzyme activites, overview Yersinia intermedia
0.0096
-
 UDP-glucose pH 7.5, 37°C Yersinia intermedia
0.0104
-
 UDP-glucose pH 7.5, 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Escherichia coli
Mg2+
-
 Yersinia intermedia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 x * 63000, band 1, SDS-PAGE, x * 23000, band 2, SDS-PAGE Yersinia intermedia
52000
-
 gel filtration Yersinia intermedia
63000
-
 x * 63000, band 1, SDS-PAGE, x * 23000, band 2, SDS-PAGE Yersinia intermedia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + H2O Yersinia intermedia
-
 UMP + alpha-D-glucose 1-phosphate
-
 ?
UDP-glucose + H2O Escherichia coli UshA protein, with 5'-nucleotidase and UDP-sugar hydrolase activities, is required for growth on AMP or UDP-glucose as sole sources of phosphorus UMP + alpha-D-glucose 1-phosphate
-
 ?
UDP-glucose + H2O Yersinia intermedia CECT 7230
-
 UMP + alpha-D-glucose 1-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 gene ushA
-
Yersinia intermedia A4URQ8 gene ushA
-
Yersinia intermedia CECT 7230 A4URQ8 gene ushA
-

Purification (Commentary)

Purification (Comment) Organism
UshA from suspension cell culture by gel filtration, dialysis, and anion exchange chromatography for enzyme characterization, and also by another adsorption chromatography step for peptide mass fingerprinting Yersinia intermedia

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture the CDP-hydrolase activity is released on Triton X-100-containing medium. Growth at 25°C is deemed optimum in terms of the enzyme-activity yield Yersinia intermedia
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme shows UDP-sugar hydrolase EC 3.6.1.45, dinucleoside-polyphosphate hydrolase, CDP-alcohol hydrolase EC 3.6.1.53, and 5'-nucleosidase activities EC 3.1.3.5, it has a variety of activities and structure-function relationships, substrate specificity, overview Escherichia coli ?
-
 ?
additional information the enzyme shows UDP-sugar hydrolase EC 3.6.1.45, dinucleoside-polyphosphate hydrolase, CDP-alcohol hydrolase EC 3.6.1.53, and 5'-nucleosidase activities EC 3.1.3.5, it has a variety of activities and structure-function relationships, substrate specificity, overview Yersinia intermedia ?
-
 ?
additional information the enzyme shows UDP-sugar hydrolase EC 3.6.1.45, dinucleoside-polyphosphate hydrolase, CDP-alcohol hydrolase EC 3.6.1.53, and 5'-nucleosidase activities EC 3.1.3.5, it has a variety of activities and structure-function relationships, substrate specificity, overview Yersinia intermedia CECT 7230 ?
-
 ?
UDP-glucose + H2O
-
 Escherichia coli UMP + alpha-D-glucose 1-phosphate
-
 ?
UDP-glucose + H2O
-
 Yersinia intermedia UMP + alpha-D-glucose 1-phosphate
-
 ?
UDP-glucose + H2O
-
 Yersinia intermedia UMP + alpha-D-glucose 1-phosphate products are uridine, phosphate and D-glucose 1-phosphate. The phosphoanhydride and the nucleoside 5'-ester linkages are hydrolyzed without detectable signs of nucleoside monophosphate as an intermediate, possibly because it is hydrolyzed as soon as it is formed without leaving the active center ?
UDP-glucose + H2O UshA protein, with 5'-nucleotidase and UDP-sugar hydrolase activities, is required for growth on AMP or UDP-glucose as sole sources of phosphorus Escherichia coli UMP + alpha-D-glucose 1-phosphate
-
 ?
UDP-glucose + H2O
-
 Yersinia intermedia CECT 7230 UMP + alpha-D-glucose 1-phosphate
-
 ?
UDP-glucose + H2O
-
 Yersinia intermedia CECT 7230 UMP + alpha-D-glucose 1-phosphate products are uridine, phosphate and D-glucose 1-phosphate. The phosphoanhydride and the nucleoside 5'-ester linkages are hydrolyzed without detectable signs of nucleoside monophosphate as an intermediate, possibly because it is hydrolyzed as soon as it is formed without leaving the active center ?

Subunits

Subunits Comment Organism
? x * 63000, band 1, SDS-PAGE, x * 23000, band 2, SDS-PAGE Yersinia intermedia
More UshA peptide mass fingerprint, overview Yersinia intermedia

Synonyms

Synonyms Comment Organism
5'-nucleotidase/UDP-sugar hydrolase
-
 Escherichia coli
5'-nucleotidase/UDP-sugar hydrolase
-
 Yersinia intermedia
UshA
-
 Escherichia coli
UshA
-
 Yersinia intermedia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli
37
-
 assay at Yersinia intermedia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
71
-
 UDP-glucose pH 7.5, 37°C Escherichia coli
97
-
 UDP-glucose pH 7.5, 37°C Yersinia intermedia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Escherichia coli
7.5
-
 assay at Yersinia intermedia