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Literature summary for 3.6.1.45 extracted from
- Cloning, expression and characterization of a mammalian Nudix hydrolase-like enzyme that cleaves the pyrophosphate bond of UDP-glucose (2003), Biochem. J., 370, 409-415.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene UGPP, overexpression in Escherichia coli strain AD494(DE3), subcloning in strain JM109 | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | complete inhibition at 1 mM | Sus scrofa |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | 100000 x g supernatant from kidney | Sus scrofa | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
| Mg2+ | absolutely required for optimal activity, not replacable by Mn2+ | Sus scrofa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
2 * 28000, SDS-PAGE | Sus scrofa |
| 50000 | 60000 | gel filtration | Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + H2O | Sus scrofa | hydrolysis of the precursor molecule of numerous glycosylation reactions in animals, to produce glucose 1-phosphate and UMP | UMP + alpha-D-glucose 1-phosphate | - |
? | |
| UDP-glucose + H2O | Homo sapiens | hydrolysis of the precursor molecule of numerous glycosylation reactions in animals, to produce glucose 1-phosphate and UMP | UMP + alpha-D-glucose 1-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O95848 | gene UGPP | - |
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from kineys by anion exchange chromatography, hydroxyapatite and again anion exchange chromatography, gel filtration, another different step of anion exchange and finally cation exchange chromatography followed by native PAGE | Homo sapiens |
| native enzyme over 20000fold from kidneys by anion exchange chromatography, hydroxyapatite and again anion exchange chromatography, gel filtration, another different step of anion exchange and finally cation exchange chromatography followed by native PAGE | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Sus scrofa | - |
| thyroid | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-glucose + H2O | 15% of the activity with UDP-glucose | Homo sapiens | AMP + alpha-D-glucose 1-phosphate | - |
? | |
| ADP-glucose + H2O | 20% of the activity with UDP-glucose | Sus scrofa | AMP + alpha-D-glucose 1-phosphate | - |
? | |
| ADP-ribose + H2O | 95% of the activity with UDP-glucose | Sus scrofa | AMP + alpha-D-ribose 1-phosphate | - |
? | |
| ADP-ribose + H2O | best substrate, 130% of the activity with UDP-glucose | Homo sapiens | AMP + alpha-D-ribose 1-phosphate | - |
? | |
| CDP-glucose + H2O | 2% of the activity with UDP-glucose | Sus scrofa | CMP + alpha-D-glucose 1-phosphate | - |
? | |
| CDP-glucose + H2O | 3% of the activity with UDP-glucose | Homo sapiens | CMP + alpha-D-glucose 1-phosphate | - |
? | |
| GDP-glucose + H2O | 10% of the activity with UDP-glucose | Sus scrofa | GMP + alpha-D-glucose 1-phosphate | - |
? | |
| GDP-glucose + H2O | 10% of the activity with UDP-glucose | Homo sapiens | GMP + alpha-D-glucose 1-phosphate | - |
? | |
| UDP-glucose + H2O | - |
Homo sapiens | UMP + alpha-D-glucose 1-phosphate | - |
? | |
| UDP-glucose + H2O | best substrate | Sus scrofa | UMP + alpha-D-glucose 1-phosphate | - |
? | |
| UDP-glucose + H2O | hydrolysis of the precursor molecule of numerous glycosylation reactions in animals, to produce glucose 1-phosphate and UMP | Sus scrofa | UMP + alpha-D-glucose 1-phosphate | - |
? | |
| UDP-glucose + H2O | hydrolysis of the precursor molecule of numerous glycosylation reactions in animals, to produce glucose 1-phosphate and UMP | Homo sapiens | UMP + alpha-D-glucose 1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 28000, SDS-PAGE | Sus scrofa |
| More | amino acid sequence comparisons, overview | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Nudix hydrolase-like nucleotide hydrolase | - |
Sus scrofa |
| Nudix hydrolase-like nucleotide hydrolase | - |
Homo sapiens |
| UDP-glucose pyrophosphatase | - |
Sus scrofa |
| UDP-glucose pyrophosphatase | - |
Homo sapiens |
| UDPG pyrophosphatase | - |
Sus scrofa |
| UDPG pyrophosphatase | - |
Homo sapiens |
| UGPPase | - |
Sus scrofa |
| UGPPase | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9.5 | with substrate UDP-glucose | Sus scrofa |
| 9 | - |
assay at | Homo sapiens |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Sus scrofa | isoelectric focusing | - |
4.7 |
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