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Literature summary for 3.6.1.44 extracted from
- Characterization of an oligosaccharide-pyrophosphodolichol pyrophosphatase activity in yeast (1988), Biochem. J., 255, 235-242.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | 6fold increase at 0.5% | Saccharomyces cerevisiae |  |
| Triton X-114 | 7fold increase at 0.5% | Saccharomyces cerevisiae | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| bacitracin | 80% inhibition at 10 mM | Saccharomyces cerevisiae | |
| diphosphate | total inhibition at 10 mM | Saccharomyces cerevisiae | |
| NAD+ | total inhibition at 10 mM | Saccharomyces cerevisiae | |
| phosphate | 35% inhibition at 10 mM | Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Saccharomyces cerevisiae | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 150% activity compared to control, 10 mM | Saccharomyces cerevisiae | |
| Mg2+ | 113% activity compared to control, 10 mM | Saccharomyces cerevisiae | |
| Mn2+ | 149.5% activity compared to control, 10 mM | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oligosaccharide-diphosphodolichol + H2O | Saccharomyces cerevisiae | - |
oligosaccharide phosphate + dolichyl phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oligosaccharide-diphosphodolichol + H2O | - |
Saccharomyces cerevisiae | oligosaccharide phosphate + dolichyl phosphate | - |
? | |
| oligosaccharide-diphosphodolichol + H2O | oligosaccharide-diphosphodolichol from mouse splenocytes | Saccharomyces cerevisiae | oligosaccharide phosphate + dolichyl phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 35 | no activity at 0°C | Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
preincubation for 10 min, half-life at 62°C 1 hour, activity lost after incubation at 70°C | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | no activity above pH 9.0 | Saccharomyces cerevisiae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Saccharomyces cerevisiae |
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