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Literature summary for 3.6.1.43 extracted from
- Brain dolichyl pyrophosphate phosphatase. Solubilization, characterization, and differentiation from dolichyl monophosphate phosphatase activity (1984), J. Biol. Chem., 259, 14580-14585.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | - |
Bos taurus |  |
| EDTA | - |
Bos taurus | |
| phosphate | - |
Bos taurus | |
| Trimetaphosphate | - |
Bos taurus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| bacitracin | complete inhibition | Bos taurus | |
| Ca2+ | - |
Bos taurus | |
| farnesol | very slight inhibition | Bos taurus | |
| farnesyl diphosphate | - |
Bos taurus | |
| isopentenyl diphosphate | - |
Bos taurus | |
| Mn2+ | strong inhibitor | Bos taurus | |
| NaF | slight inhibition | Bos taurus | |
| phosphate | activating | Bos taurus | |
| undecaprenyl diphosphate | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
dolichyl diphosphate | - |
Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | integral | Bos taurus | 16020 | - |
| microsome | - |
Bos taurus | - |
- |
| mitochondrial outer membrane | integral | Bos taurus | 5741 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | inhibitory | Bos taurus | |
| Mn2+ | strong inhibitor | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dolichyl diphosphate + H2O | Bos taurus | involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate | dolichyl phosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0031 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -17°C stable one month | Bos taurus |
| 4°C solubilized enzyme stable overnight | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dolichyl diphosphate + H2O | - |
Bos taurus | dolichyl phosphate + phosphate | - |
? | |
| dolichyl diphosphate + H2O | involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate | Bos taurus | dolichyl phosphate + phosphate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
2 min causes 80-85% loss of activity | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
optimum activity at neutral pH | Bos taurus |
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Release 2023.1 (January 2023)
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