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Literature summary for 3.6.1.42 extracted from

  • Raychaudhuri, P.; Ghosh, S.; Maitra, U.
    Purification and characterization of a guanosine diphosphatase activity from calf liver microsomal salt wash proteins (1985), J. Biol. Chem., 260, 8306-8311.
    View publication on PubMed
Search for more literature for 3.6.1.42

Inhibitors

Inhibitors Comment Organism Structure
GDP hydrolysis of UDP Bos taurus
UDP hydrolysis of GDP Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Bos taurus
-
-
ribosome
-
 Bos taurus 5840
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 calf, gel filtration and glycerol density gradient centrifugation Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP + H2O Bos taurus possible biological function in maintaining the cellular pool of GTP-GDP 5'-GMP + phosphate
-
 ?
GDP + H2O Bos taurus GTP is an important steric effector in many biological systems, GDP a potent inhibitor: GDPase activity could be involved in preventing potential inhibitory levels of GDP from accumulating 5'-GMP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 calf
-

Purification (Commentary)

Purification (Comment) Organism
calf Bos taurus

Reaction

Reaction Comment Organism Reaction ID
GDP + H2O = GMP + phosphate stoichiometry of reaction Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bos taurus
4000
-
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP + H2O best substrate Bos taurus 5'-GMP + phosphate
-
 ?
GDP + H2O hydrolyzes free GDP, unable to hydrolyze GDP bound to eIF-2, eukaryotic initiation factor 2, and unable to catalyze the exchange of bound GDP with GTP even in the presence of relatively high molar excess of GTP and Met-tRNA Bos taurus 5'-GMP + phosphate
-
 ?
GDP + H2O possible biological function in maintaining the cellular pool of GTP-GDP Bos taurus 5'-GMP + phosphate
-
 ?
GDP + H2O GTP is an important steric effector in many biological systems, GDP a potent inhibitor: GDPase activity could be involved in preventing potential inhibitory levels of GDP from accumulating Bos taurus 5'-GMP + phosphate
-
 ?
additional information substrate specificity Bos taurus ?
-
 ?
additional information hydrolyzes free GDP, unable to hydrolyze GDP bound to eIF-2, eukaryotic initiation factor 2, and unable to catalyze the exchange of bound GDP with GTP even in the presence of relatively high molar excess of GTP and Met-tRNA Bos taurus ?
-
 ?
additional information only GDP and UDP are hydrolyzed, no activity with other nucleoside diphosphates and triphosphates, no activity with CDP, ADP, GTP, ATP Bos taurus ?
-
 ?
UDP + H2O much lower affinity than for GDP Bos taurus UMP + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 46000, calf, SDS-PAGE Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Bos taurus