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Literature summary for 3.6.1.40 extracted from

  • Hara, A.; Sy, J.
    Guanosine 5'-triphosphate, 3'-diphosphate 5'-phosphohydrolase. Purification and substrate specificity (1983), J. Biol. Chem., 258, 1678-1683.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
guanosine 5'-triphosphate,3'-diphosphate
-
Escherichia coli
0.13
-
Guanosine 5'-triphosphate,3'-monophosphate
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
K+ monovalent cation required, NH4+ preferred over K+, Na+ ineffective Escherichia coli
Mg2+ required, optimum activity above 1 mM Escherichia coli
NH4+ monovalent cation required, NH4+ preferred over K+, Na+ ineffective Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
guanosine 5'-triphosphate,3'-diphosphate + H2O Escherichia coli involved in nutritional stress response via ppGpp guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
?
guanosine 5'-triphosphate,3'-diphosphate + H2O Escherichia coli JF1599 involved in nutritional stress response via ppGpp guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli JF1599
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.08
-
crude extract Escherichia coli
1513
-
partially purified enzyme Escherichia coli

Storage Stability

Storage Stability Organism
unstable at 4°C, storable in liquid nitrogen Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanosine 5'-triphosphate,3'-diphosphate + H2O
-
Escherichia coli guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
?
guanosine 5'-triphosphate,3'-diphosphate + H2O involved in nutritional stress response via ppGpp Escherichia coli guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
?
guanosine 5'-triphosphate,3'-diphosphate + H2O
-
Escherichia coli JF1599 guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
?
guanosine 5'-triphosphate,3'-diphosphate + H2O involved in nutritional stress response via ppGpp Escherichia coli JF1599 guanosine 5'-diphosphate,3'-diphosphate + phosphate
-
?
guanosine 5'-triphosphate,3'-monophosphate + H2O
-
Escherichia coli guanosine 5'-diphosphate,3'-monophosphate + phosphate
-
?
guanosine 5'-triphosphate,3'-monophosphate + H2O
-
Escherichia coli JF1599 guanosine 5'-diphosphate,3'-monophosphate + phosphate
-
?
additional information not: GTP, ATP or adenosine 5'-triphosphate,3'-diphosphate, guanosine 5'-diphosphate,3'-diphosphate, guanosine 5'-triphosphate,3'-monophosphate-nucleotide, enzyme is rather nonspecific towards the 3'-OH substitutions of the substrates although a free, unsubstituted phosphate group at the 3'-OH position is essential Escherichia coli ?
-
?
additional information not: GTP, ATP or adenosine 5'-triphosphate,3'-diphosphate, guanosine 5'-diphosphate,3'-diphosphate, guanosine 5'-triphosphate,3'-monophosphate-nucleotide, enzyme is rather nonspecific towards the 3'-OH substitutions of the substrates although a free, unsubstituted phosphate group at the 3'-OH position is essential Escherichia coli JF1599 ?
-
?
pppGpNp + H2O cleavage of 5'-phosphate ends of RNA Escherichia coli ppGpNp + phosphate
-
?
pppGpNp + H2O cleavage of 5'-phosphate ends of RNA Escherichia coli JF1599 ppGpNp + phosphate
-
?
pppGpNp-RNA + H2O cleavage of 5'-phosphate ends of RNA, poor substrate Escherichia coli ppGpNp-RNA + phosphate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
optimum activity in borate buffer Escherichia coli