KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
guanosine 5'-triphosphate,3'-diphosphate | - |
Escherichia coli | |
0.13 | - |
Guanosine 5'-triphosphate,3'-monophosphate | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | monovalent cation required, NH4+ preferred over K+, Na+ ineffective | Escherichia coli | |
Mg2+ | required, optimum activity above 1 mM | Escherichia coli | |
NH4+ | monovalent cation required, NH4+ preferred over K+, Na+ ineffective | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanosine 5'-triphosphate,3'-diphosphate + H2O | Escherichia coli | involved in nutritional stress response via ppGpp | guanosine 5'-diphosphate,3'-diphosphate + phosphate | - |
? | |
guanosine 5'-triphosphate,3'-diphosphate + H2O | Escherichia coli JF1599 | involved in nutritional stress response via ppGpp | guanosine 5'-diphosphate,3'-diphosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli JF1599 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.08 | - |
crude extract | Escherichia coli |
1513 | - |
partially purified enzyme | Escherichia coli |
Storage Stability | Organism |
---|---|
unstable at 4°C, storable in liquid nitrogen | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanosine 5'-triphosphate,3'-diphosphate + H2O | - |
Escherichia coli | guanosine 5'-diphosphate,3'-diphosphate + phosphate | - |
? | |
guanosine 5'-triphosphate,3'-diphosphate + H2O | involved in nutritional stress response via ppGpp | Escherichia coli | guanosine 5'-diphosphate,3'-diphosphate + phosphate | - |
? | |
guanosine 5'-triphosphate,3'-diphosphate + H2O | - |
Escherichia coli JF1599 | guanosine 5'-diphosphate,3'-diphosphate + phosphate | - |
? | |
guanosine 5'-triphosphate,3'-diphosphate + H2O | involved in nutritional stress response via ppGpp | Escherichia coli JF1599 | guanosine 5'-diphosphate,3'-diphosphate + phosphate | - |
? | |
guanosine 5'-triphosphate,3'-monophosphate + H2O | - |
Escherichia coli | guanosine 5'-diphosphate,3'-monophosphate + phosphate | - |
? | |
guanosine 5'-triphosphate,3'-monophosphate + H2O | - |
Escherichia coli JF1599 | guanosine 5'-diphosphate,3'-monophosphate + phosphate | - |
? | |
additional information | not: GTP, ATP or adenosine 5'-triphosphate,3'-diphosphate, guanosine 5'-diphosphate,3'-diphosphate, guanosine 5'-triphosphate,3'-monophosphate-nucleotide, enzyme is rather nonspecific towards the 3'-OH substitutions of the substrates although a free, unsubstituted phosphate group at the 3'-OH position is essential | Escherichia coli | ? | - |
? | |
additional information | not: GTP, ATP or adenosine 5'-triphosphate,3'-diphosphate, guanosine 5'-diphosphate,3'-diphosphate, guanosine 5'-triphosphate,3'-monophosphate-nucleotide, enzyme is rather nonspecific towards the 3'-OH substitutions of the substrates although a free, unsubstituted phosphate group at the 3'-OH position is essential | Escherichia coli JF1599 | ? | - |
? | |
pppGpNp + H2O | cleavage of 5'-phosphate ends of RNA | Escherichia coli | ppGpNp + phosphate | - |
? | |
pppGpNp + H2O | cleavage of 5'-phosphate ends of RNA | Escherichia coli JF1599 | ppGpNp + phosphate | - |
? | |
pppGpNp-RNA + H2O | cleavage of 5'-phosphate ends of RNA, poor substrate | Escherichia coli | ppGpNp-RNA + phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
optimum activity in borate buffer | Escherichia coli |