Literature summary for 3.6.1.29 extracted from

Garrison, P.N.; Robinson, A.K.; Pekarsky, Y.; Croce, C.M.; Barnes, L.D.
Phosphorylation of the human Fhit tumor suppressor on tyrosine 114 in Escherichia coli and unexpected steady state kinetics of the phosphorylated forms (2005), Biochemistry, 44, 6286-6292.

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Cloned(Commentary)

Cloned (Comment)	Organism
co-expression of Fhit with the elk tyrosine kinase in Escherichia coli strain TBK1, expression of nonphosphorylized enzyme in Escherichia coli strain SG100	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	steady state kinetics of the phosphorylated enzyme form	Homo sapiens
0.00066	-	P1,P3-bis(5'-adenosyl)triphosphate	diphosphorylated recombinant enzyme, pH 6.8, 37°C	Homo sapiens
0.00067	-	P1,P3-bis(5'-adenosyl)triphosphate	monophosphorylated recombinant enzyme, pH 6.8, 37°C	Homo sapiens
0.0016	-	P1,P3-bis(5'-adenosyl)triphosphate	nonphosphorylated recombinant enzyme, pH 6.8, 37°C	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	dependent on	Homo sapiens
Mn2+	activates	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
P1,P3-bis(5'-adenosyl)triphosphate + H2O	Homo sapiens	-	ADP + AMP	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P49789	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	Fhit is phosphorylated in vivo, and is phosphorylated in vitro by Src kinase	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
co-expression of Fhit with the elk tyrosine kinase in Escherichia coli strain TBK1 by ion exchange chromatography, gel filtration, and another step of ion exchange chromatography	Homo sapiens

Reaction

Reaction	Comment	Organism	Reaction ID
P1,P3-bis(5'-adenosyl) triphosphate + H2O = ADP + AMP	the dimeric enzyme contains a catalytic histidine triad with H96 forming a covalent intermediate at the alpha-phosphate of P1,P3-bis(5'-adenosyl)triphosphate, S128, H96 and H98 are essential for the reaction, structure overview	Homo sapiens	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
P1,P3-bis(5'-adenosyl)triphosphate + H2O	-	Homo sapiens	ADP + AMP	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Homo sapiens

Synonyms

Synonyms	Comment	Organism
Fhit	-	Homo sapiens
fragile histidine triad	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.02	-	P1,P3-bis(5'-adenosyl)triphosphate	diphosphorylated recombinant enzyme, pH 6.8, 37°C	Homo sapiens
0.38	-	P1,P3-bis(5'-adenosyl)triphosphate	monophosphorylated recombinant enzyme, pH 6.8, 37°C	Homo sapiens
2.74	-	P1,P3-bis(5'-adenosyl)triphosphate	nonphosphorylated recombinant enzyme, pH 6.8, 37°C	Homo sapiens
3	6	P1,P3-bis(5'-adenosyl)triphosphate	nonphosphorylated recombinant enzyme, pH 6.8, 37°C	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.8	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)