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Literature summary for 3.6.1.29 extracted from

  • Prescott, M.; Thorne, N.M.H.; Milne, A.D.; McLennan, A.G.
    Characterization of a bis(5'-nucleosidyl) triphosphate pyrophosphohydrolase from encysted embryos of the brine shrimp Artemia (1992), Int. J. Biochem., 24, 565-571.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
Artemia sp.
Ni2+
-
Artemia sp.
Zn2+
-
Artemia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
P1-P3-bis(5'-guanosyl) triphosphate
-
Artemia sp.
0.015
-
P1-P3-bis(5'-adenosyl) triphosphate
-
Artemia sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Artemia sp.
Mg2+
-
Artemia sp.
Mn2+
-
Artemia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
gel filtration Artemia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
P1-P3-bis(5'-adenosyl) triphosphate + H2O Artemia sp.
-
ADP + AMP
-
?

Organism

Organism UniProt Comment Textmining
Artemia sp.
-
shrimp
-

Purification (Commentary)

Purification (Comment) Organism
partially Artemia sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0075
-
-
Artemia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
-
Artemia sp. AMP + ATP
-
?
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
-
Artemia sp. AMP + adenosine 5'-tetraphosphate
-
?
Gp4A + H2O
-
Artemia sp. ?
-
?
Gp5A + H2O
-
Artemia sp. ?
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
-
Artemia sp. ADP + AMP
-
?
P1-P3-bis(5'-guanosyl) triphosphate + H2O
-
Artemia sp. GDP + GMP
-
?
P1-P3-bis(5'-uracyl) triphosphate + H2O
-
Artemia sp. UDP + UMP
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Artemia sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45
-
Artemia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Artemia sp.