Literature summary for 3.6.1.28 extracted from

Lakaye, B.; Makarchikov, A.F.; Wins, P.; Margineanu, I.; Roland, S.; Lins, L.; Aichour, R.; Lebeau, L.; El Moualij, B.; Zorzi, W.; Coumans, B.; Grisar, T.; Bettendorff, L.
Human recombinant thiamine triphosphatase: purification, secondary structure and catalytic properties (2004), Int. J. Biochem. Cell Biol., 36, 1348-1364.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli, the recombinant enzyme is completely functional	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
([2-[3-(4-Amino-2-methyl-pyrimidin-5-ylmethyl)-4-methyl-thiazol-5-yl]-ethoxy]-phosphonomethyl-phosphinoylmethyl)-phosphonic acid	-	Homo sapiens
ATP	very poor inhibitor	Homo sapiens
Ca2+	inhibits by competition with Mg2+	Homo sapiens
SDS	IC50: about 0.3% W/v. The inhibition is partially reversible, probably due to correct refolding of the denatured enzyme	Homo sapiens
thiamine triphosphate	-	Homo sapiens
Zn2+	inhibits at micromolar concentrations at pH 8.0, IC50: about 0.015-0.02 mM. Activates at pH 6.0	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.154	-	thiamine triphosphate	untagged recombinant enzyme	Homo sapiens
3.7	-	ATP	in presence of 8 mM ATP	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
soluble	-	Homo sapiens	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Homo sapiens
Zn2+	inhibits at micromolar concentrations at pH 8.0, activates at pH 6.0	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
140	-	purified recombinant enzyme	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O	the catalytic efficiency is about 4 orders of magnitude lower than for thiamine triphosphate	Homo sapiens	ADP + phosphate	-	?
thiamine triphosphate + H2O	-	Homo sapiens	thiamine diphosphate + phosphate	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
55	60	30 min, stable	Homo sapiens
68	-	30 min, 50% loss of activity	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.24	-	ATP	in presence of 8 mM Mg2+	Homo sapiens
140	-	thiamine triphosphate	-	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Homo sapiens

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	9.5	pH 7.0: about 60% of maximal activity, pH 9.5: about 75% of maximal activity	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.95	-	thiamine triphosphate	-	Homo sapiens
1.2	-	([2-[3-(4-Amino-2-methyl-pyrimidin-5-ylmethyl)-4-methyl-thiazol-5-yl]-ethoxy]-phosphonomethyl-phosphinoylmethyl)-phosphonic acid	-	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.015	0.02	inhibits at micromolar concentrations at pH 8.0, IC50: about 0.015-0.02 mM. Activates at pH 6.0	Homo sapiens	Zn2+

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Release 2023.1 (January 2023)