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Literature summary for 3.6.1.24 extracted from

  • Spahr, P.F.; Gesteland, R.F.
    Purification and properties of a new enzyme from Escherichia coli: nucleoside phosphacyl hydrolase (1970), Eur. J. Biochem., 12, 270-284.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Li2SO4 160% of standard activity at 0.75 M Escherichia coli
NH4Cl 180% of standard activity at 1 M Escherichia coli

General Stability

General Stability Organism
inactivation by repeated freezing and thawing Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
alanyl adenylate competitive inhibition of other substrates Escherichia coli
aminoalkyl adenylate strongest inhibitor, competitive inhibition of other substrates Escherichia coli
AMP competitive Escherichia coli
KI 50% loss of activity at 1 M Escherichia coli
lysyl adenylate competitive inhibition of other substrates Escherichia coli
Methionyl adenylate competitive inhibition of other substrates Escherichia coli
SDS complete loss of activity at 6 mM Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.056
-
adenosine 5'-p-nitrophenylphosphate
-
Escherichia coli
0.11
-
guanosine 5'-p-nitrophenylphosphate
-
Escherichia coli
0.65
-
uridine 5'-p-nitrophenylphosphate
-
Escherichia coli
4
-
cytidine 5'-p-nitrophenylphosphate
-
Escherichia coli
770
-
thymidine 5'-p-nitrophenylphosphate
-
Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information not periplasm Escherichia coli
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal ions required Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
sedimentation equilibrium method, gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
p-nitrophenyl 5'-nucleotides + H2O Escherichia coli hydrolysis A,C,G,U,T nucleotides p-nitrophenol + nucleoside phosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Oxidation Stability

Oxidation Stability Organism
stable towards reducing agents Escherichia coli

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Renatured (Commentary)

Renatured (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0018
-
crude extract Escherichia coli
6670
-
purified enzyme Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, can be stored for at least one year Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl adenylate + H2O
-
Escherichia coli ?
-
?
adenosine 5'-p-nitrophenylphosphate + H2O
-
Escherichia coli p-nitrophenol + AMP
-
?
cytidine 5'-p-nitrophenylphosphate + H2O
-
Escherichia coli p-nitrophenol + CMP
-
?
guanosine 5'-p-nitrophenylphosphate + H2O
-
Escherichia coli p-nitrophenol + GMP
-
?
methionyl adenylate + H2O
-
Escherichia coli ?
-
?
p-nitrophenyl 5'-nucleotides + H2O hydrolysis A,C,G,U,T nucleotides Escherichia coli p-nitrophenol + nucleoside phosphate
-
?
thymidine 5'-p-nitrophenylphosphate + H2O
-
Escherichia coli p-nitrophenol + TMP
-
?
uridine 5'-p-nitrophenylphosphate + H2O
-
Escherichia coli p-nitrophenol + UMP
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Escherichia coli

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 65 rapid loss of activity above 50°C Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
no loss of activity within one day Escherichia coli
65
-
loss of activity above 65°C, can be restored by cooling down to 0°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
5.5 10.5 very low activity at pH 5.5 and 10.5 Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5
-
irreversible inactivation below pH 7.5 Escherichia coli