Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Li2SO4 | 160% of standard activity at 0.75 M | Escherichia coli | |
NH4Cl | 180% of standard activity at 1 M | Escherichia coli |
General Stability | Organism |
---|---|
inactivation by repeated freezing and thawing | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alanyl adenylate | competitive inhibition of other substrates | Escherichia coli | |
aminoalkyl adenylate | strongest inhibitor, competitive inhibition of other substrates | Escherichia coli | |
AMP | competitive | Escherichia coli | |
KI | 50% loss of activity at 1 M | Escherichia coli | |
lysyl adenylate | competitive inhibition of other substrates | Escherichia coli | |
Methionyl adenylate | competitive inhibition of other substrates | Escherichia coli | |
SDS | complete loss of activity at 6 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.056 | - |
adenosine 5'-p-nitrophenylphosphate | - |
Escherichia coli | |
0.11 | - |
guanosine 5'-p-nitrophenylphosphate | - |
Escherichia coli | |
0.65 | - |
uridine 5'-p-nitrophenylphosphate | - |
Escherichia coli | |
4 | - |
cytidine 5'-p-nitrophenylphosphate | - |
Escherichia coli | |
770 | - |
thymidine 5'-p-nitrophenylphosphate | - |
Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | not periplasm | Escherichia coli | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal ions required | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
sedimentation equilibrium method, gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl 5'-nucleotides + H2O | Escherichia coli | hydrolysis A,C,G,U,T nucleotides | p-nitrophenol + nucleoside phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Oxidation Stability | Organism |
---|---|
stable towards reducing agents | Escherichia coli |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Renatured (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0018 | - |
crude extract | Escherichia coli |
6670 | - |
purified enzyme | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, can be stored for at least one year | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl adenylate + H2O | - |
Escherichia coli | ? | - |
? | |
adenosine 5'-p-nitrophenylphosphate + H2O | - |
Escherichia coli | p-nitrophenol + AMP | - |
? | |
cytidine 5'-p-nitrophenylphosphate + H2O | - |
Escherichia coli | p-nitrophenol + CMP | - |
? | |
guanosine 5'-p-nitrophenylphosphate + H2O | - |
Escherichia coli | p-nitrophenol + GMP | - |
? | |
methionyl adenylate + H2O | - |
Escherichia coli | ? | - |
? | |
p-nitrophenyl 5'-nucleotides + H2O | hydrolysis A,C,G,U,T nucleotides | Escherichia coli | p-nitrophenol + nucleoside phosphate | - |
? | |
thymidine 5'-p-nitrophenylphosphate + H2O | - |
Escherichia coli | p-nitrophenol + TMP | - |
? | |
uridine 5'-p-nitrophenylphosphate + H2O | - |
Escherichia coli | p-nitrophenol + UMP | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 65 | rapid loss of activity above 50°C | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
no loss of activity within one day | Escherichia coli |
65 | - |
loss of activity above 65°C, can be restored by cooling down to 0°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10.5 | very low activity at pH 5.5 and 10.5 | Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
irreversible inactivation below pH 7.5 | Escherichia coli |