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Literature summary for 3.6.1.18 extracted from
- Studies on nucleotidases in plants: Part II - Isolation and properties of an enzyme hydrolyzing FAD at alkaline pH from mung bean seedlings (Phaseolus radiatus) (1968), Indian J. Biochem., 5, 137-142.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Vigna radiata var. radiata |  |
| 2-mercaptoethanol | - |
Vigna radiata var. radiata | |
| 8-hydroxyquinoline | - |
Vigna radiata var. radiata | |
| diphosphate | - |
Vigna radiata var. radiata | |
| EDTA | inhibitory | Vigna radiata var. radiata | |
| GSH | - |
Vigna radiata var. radiata | |
| molybdate | slight inhibition at 1 mM | Vigna radiata var. radiata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
NADH | - |
Vigna radiata var. radiata | |
| 0.33 | - |
FAD | - |
Vigna radiata var. radiata | |
| 0.58 | - |
NAD+ | - |
Vigna radiata var. radiata | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | inhibitory at high concentrations | Vigna radiata var. radiata | |
| Co2+ | partially reverses EDTA inhibition, activating | Vigna radiata var. radiata | |
| Cu2+ | inhibitory at high concentrations | Vigna radiata var. radiata | |
| Hg2+ | inhibitory at high concentrations | Vigna radiata var. radiata | |
| Ni2+ | inhibitory at high concentrations | Vigna radiata var. radiata | |
| Zn2+ | reverses EDTA inhibition, inhibitory at concentrations above 0.1 mM | Vigna radiata var. radiata | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| FAD + H2O | Vigna radiata var. radiata | - |
FMN + AMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vigna radiata var. radiata | - |
mung bean seedlings | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| stable at 0-4°C | Vigna radiata var. radiata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| FAD + H2O | - |
Vigna radiata var. radiata | FMN + AMP | - |
? | |
| NAD+ + H2O | - |
Vigna radiata var. radiata | NMN + AMP | - |
? | |
| NADH + H2O | - |
Vigna radiata var. radiata | NMNH + AMP | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 49 | 51 | - |
Vigna radiata var. radiata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.4 | - |
- |
Vigna radiata var. radiata |
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