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Literature summary for 3.6.1.17 extracted from

  • Barta, M.L.; Lovell, S.; Sinclair, A.N.; Battaile, K.P.; Hefty, P.S.
    Chlamydia trachomatis CT771 (nudH) is an asymmetric Ap4A hydrolase (2014), Biochemistry, 53, 214-224.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.6.1.17

Crystallization (Commentary)

Crystallization (Comment) Organism
structures of both apo- and ligand-bound CT771, to 2.6 A and 1.9 A resolution, respectively. The structure shows a alphabetaalpha-sandwich motif with many conserved elements lining the putative Nudix active site Chlamydia trachomatis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 diadenosine 5',5''-P1,P4-tetraphosphate presence of 5.0 mM MnCl2, pH 7.5, 22°C Chlamydia trachomatis
0.0073
-
 diadenosine 5',5''-P1,P4-tetraphosphate presence of 1.5 mM MnCl2, pH 7.5, 22°C Chlamydia trachomatis
0.0093
-
 diadenosine 5',5''-P1,P4-tetraphosphate presence of 3.0 mM MnCl2, pH 7.5, 22°C Chlamydia trachomatis
0.326
-
 diadenosine 5',5''-P1,P4-tetraphosphate presence of 3.0 mM MgCl2, pH 7.5, 22°C Chlamydia trachomatis
0.333
-
 diadenosine 5',5''-P1,P4-tetraphosphate presence of 5.0 mM MgCl2, pH 7.5, 22°C Chlamydia trachomatis
0.407
-
 diadenosine 5',5''-P1,P4-tetraphosphate presence of 1.5 mM MgCl2, pH 7.5, 22°C Chlamydia trachomatis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ in the presence of Mn2+ the Km value for diadenosine 5',5''-P1,P4-tetraphosphate is roughly two orders of magnitude tighter than in the presence of Mg2+ Chlamydia trachomatis
Mn2+ in the presence of Mn2+ the Km value for diadenosine 5',5''-P1,P4-tetraphosphate is roughly two orders of magnitude tighter than in the presence of Mg2+ Chlamydia trachomatis

Organism

Organism UniProt Comment Textmining
Chlamydia trachomatis
-
 serovar 434/Bu
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
-
 Chlamydia trachomatis ATP + AMP
-
 ?

Synonyms

Synonyms Comment Organism
CT771
-
 Chlamydia trachomatis
nudH
-
 Chlamydia trachomatis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.68
-
 diadenosine 5',5''-P1,P4-tetraphosphate presence of 5.0 mM MnCl2, pH 7.5, 22°C Chlamydia trachomatis
1.51
-
 diadenosine 5',5''-P1,P4-tetraphosphate presence of 3.0 mM MnCl2, pH 7.5, 22°C Chlamydia trachomatis
1.6
-
 diadenosine 5',5''-P1,P4-tetraphosphate presence of 1.5 mM MnCl2, pH 7.5, 22°C Chlamydia trachomatis
1.78
-
 diadenosine 5',5''-P1,P4-tetraphosphate presence of 1.5 mM MgCl2, pH 7.5, 22°C Chlamydia trachomatis
2.24
-
 diadenosine 5',5''-P1,P4-tetraphosphate presence of 3.0 mM MgCl2, pH 7.5, 22°C Chlamydia trachomatis
3.25
-
 diadenosine 5',5''-P1,P4-tetraphosphate presence of 5.0 mM MgCl2, pH 7.5, 22°C Chlamydia trachomatis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.4
-
 diadenosine 5',5''-P1,P4-tetraphosphate presence of 1.5 mM MgCl2, pH 7.5, 22°C Chlamydia trachomatis
6.9
-
 diadenosine 5',5''-P1,P4-tetraphosphate presence of 3.0 mM MgCl2, pH 7.5, 22°C Chlamydia trachomatis
9.8
-
 diadenosine 5',5''-P1,P4-tetraphosphate presence of 5.0 mM MgCl2, pH 7.5, 22°C Chlamydia trachomatis
140
-
 diadenosine 5',5''-P1,P4-tetraphosphate presence of 5.0 mM MnCl2, pH 7.5, 22°C Chlamydia trachomatis
160
-
 diadenosine 5',5''-P1,P4-tetraphosphate presence of 3.0 mM MnCl2, pH 7.5, 22°C Chlamydia trachomatis
220
-
 diadenosine 5',5''-P1,P4-tetraphosphate presence of 1.5 mM MnCl2, pH 7.5, 22°C Chlamydia trachomatis