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Literature summary for 3.6.1.17 extracted from

  • Arczewska, K.D.; Baumeier, C.; Kassahun, H.; Sengupta, T.; Bjoras, M.; Kusmierek, J.T.; Nilsen, H.
    Caenorhabditis elegans NDX-4 is a MutT-type enzyme that contributes to genomic stability (2011), DNA Repair, 10, 176-187.
    View publication on PubMed
Search for more literature for 3.6.1.17

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)T cells Caenorhabditis elegans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 diadenosine 5',5''''-P1,P4-tetraphosphate in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans
0.0986
-
 8-oxo-dGTP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans
0.1143
-
 dATP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans
0.182
-
 dGTP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans
0.3846
-
 2-oxo-dATP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans Q9U2M7
-
-
Caenorhabditis elegans Bristol N2 Q9U2M7
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap FF column chromatography and Superdex 75 gel filtration Caenorhabditis elegans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-dATP + H2O less efficient hydrolysis compared to 8-oxo-dGTP Caenorhabditis elegans ?
-
 ?
2-oxo-dATP + H2O less efficient hydrolysis compared to 8-oxo-dGTP Caenorhabditis elegans Bristol N2 ?
-
 ?
8-oxo-dGTP + H2O the enzyme does not show a strong preference for 8-oxo-dGTP and is able to hydrolyze unmodified dATP and dGTP with comparable efficiency Caenorhabditis elegans 8-oxo-dGMP + diphosphate
-
 ?
8-oxo-dGTP + H2O the enzyme does not show a strong preference for 8-oxo-dGTP and is able to hydrolyze unmodified dATP and dGTP with comparable efficiency Caenorhabditis elegans Bristol N2 8-oxo-dGMP + diphosphate
-
 ?
dATP + H2O
-
 Caenorhabditis elegans ?
-
 ?
dATP + H2O
-
 Caenorhabditis elegans Bristol N2 ?
-
 ?
dGTP + H2O
-
 Caenorhabditis elegans ?
-
 ?
dGTP + H2O
-
 Caenorhabditis elegans Bristol N2 ?
-
 ?
diadenosine 5',5''''-P1,P4-tetraphosphate + H2O
-
 Caenorhabditis elegans AMP + ATP
-
 ?
diadenosine 5',5''''-P1,P4-tetraphosphate + H2O
-
 Caenorhabditis elegans Bristol N2 AMP + ATP
-
 ?

Synonyms

Synonyms Comment Organism
NDX-4
-
 Caenorhabditis elegans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.37
-
 dATP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans
0.51
-
 2-oxo-dATP in 5 0mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans
0.53
-
 dGTP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans
0.58
-
 8-oxo-dGTP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans
27
-
 diadenosine 5',5''''-P1,P4-tetraphosphate in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans

General Information

General Information Comment Organism
malfunction loss of NDX-4 leads to upregulation of key stress responsive genes that likely compensate for the in vivo role of NDX-4 in protection against deleterious consequences of oxidative stress. NDX-4 deficiency protects nematodes from acute heat shock Caenorhabditis elegans
physiological function the enzyme contributes to genomic stability in vivo in Caenorhabditis elegans Caenorhabditis elegans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3
-
 2-oxo-dATP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans
2.9
-
 dGTP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans
3.3
-
 dATP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans
5.9
-
 8-oxo-dGTP in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans
3900
-
 diadenosine 5',5''''-P1,P4-tetraphosphate in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C Caenorhabditis elegans