Activating Compound | Comment | Organism | Structure |
---|---|---|---|
nitric oxide | NO stimulates non-enzymatic ADP-ribosylation, at cysteine residues in the presence of reductant, of NUDT5 using ADP-ribose and consequently activates its ADPRase activity. ADPRase activity in J774 macrophage cells is increased by the treatment with SNP, an exogenous NO generator, or TNF-alpha/IFN-gamma, endogenous NO inducers. NO has a regulatory role, overview | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene NUDT5, expression in murine J774A.1 macrophage cells, expression of His6-tagged NUDT5 in Escherichia coli strain BL21 | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-ribose + H2O | Homo sapiens | - |
AMP + D-ribose 5-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
gene NUDT5 | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged NUDT5 from Escherichia coli strain BL21 by nickel affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,Nepsilon-etheno-ADP-ribose + H2O | fluorogenic substrate | Homo sapiens | 1,Nepsilon-etheno-AMP-ribose + phosphate | the product is converted to fluorescent 1,Nepsilon-etheno-adenosine by alkaline phosphatase for detection | ? | |
ADP-ribose + H2O | - |
Homo sapiens | AMP + D-ribose 5-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADP-ribose pyrophosphatase | - |
Homo sapiens |
ADPR pyrophosphatase | - |
Homo sapiens |
ADPRase | - |
Homo sapiens |
ADPRase-I | - |
Homo sapiens |
More | the enzyme is a member of a superfamily of Nudix hydrolases | Homo sapiens |
NUDT5 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |