Crystallization (Comment) | Organism |
---|---|
Zn2+-bound enzyme, binary complex with ADPribose, ternary complex with Zn2+ and ADPribose, ternary complex with Gd3+ and ADPribose, product complex with AMP and Mg2+, product complex with ribose 5'-phosphate and Zn2+, mutant enzyme E82Q with ligands Mg2+ and SO42-, mutant enzyme E86Q with ligands Mg2+ and ADPribose, mutant enzyme E82Q with ligands Zn2+ and SO42-, mutant enzyme E86Q with ligands Zn2+ and ADPribose | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
D126N | the ratio of turnover-number to Km-value with ADPribose as substrate and Mg2+ as activator is 40% of the of the wild-type enzyme | Thermus thermophilus |
D128N | the ratio of turnover-number to Km-value with ADPribose as substrate and Mg2+ as activator is 74% of the of the wild-type enzyme | Thermus thermophilus |
E127Q | the ratio of turnover-number to Km-value with ADPribose as substrate and Mg2+ as activator is 114% of the of the wild-type enzyme | Thermus thermophilus |
E129Q | the ratio of turnover-number to Km-value with ADPribose as substrate and Mg2+ as activator is 32% of the of the wild-type enzyme | Thermus thermophilus |
E82Q | the ratio of turnover-number to Km-value with ADPribose as substrate and Mg2+ as activator is 0.0006% of the of the wild-type enzyme | Thermus thermophilus |
E86Q | the ratio of turnover-number to Km-value with ADPribose as substrate and Mg2+ as activator is 0.0034% of the of the wild-type enzyme | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
ADPribose | mutant enzyme D126N, in presence of Mg2+ | Thermus thermophilus | |
0.044 | - |
ADPribose | mutant enzyme E129Q, in presence of Mg2+ | Thermus thermophilus | |
0.058 | - |
ADPribose | mutant enzyme D128N, in presence of Mg2+ | Thermus thermophilus | |
0.071 | - |
ADPribose | mutant enzyme E127Q, in presence of Mg2+ | Thermus thermophilus | |
0.09 | - |
ADPribose | mutant enzyme E86Q, in presence of Mg2+ | Thermus thermophilus | |
0.11 | - |
ADPribose | wild-type enzyme, in presence of Mg2+ | Thermus thermophilus | |
0.215 | - |
ADPribose | mutant enzyme E82Q, in presence of Zn2+ | Thermus thermophilus | |
0.325 | - |
ADPribose | mutant enzyme E86Q, in presence of Zn2+ | Thermus thermophilus | |
0.36 | - |
ADPribose | mutant enzyme E82Q, in presence of Mg2+ | Thermus thermophilus | |
0.37 | - |
ADPribose | wild-type enzyme, in presence of Zn2+ | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-ribose + H2O | Thermus thermophilus | the enzyme plays a key role in regulating the intracellular ADPribose levels, and prevents nonenzymatic ADP-ribosylation | AMP + D-ribose 5-phosphate | - |
? | |
ADP-ribose + H2O | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | the enzyme plays a key role in regulating the intracellular ADPribose levels, and prevents nonenzymatic ADP-ribosylation | AMP + D-ribose 5-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-ribose + H2O | - |
Thermus thermophilus | AMP + D-ribose 5-phosphate | - |
? | |
ADP-ribose + H2O | the enzyme plays a key role in regulating the intracellular ADPribose levels, and prevents nonenzymatic ADP-ribosylation | Thermus thermophilus | AMP + D-ribose 5-phosphate | - |
? | |
ADP-ribose + H2O | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + D-ribose 5-phosphate | - |
? | |
ADP-ribose + H2O | the enzyme plays a key role in regulating the intracellular ADPribose levels, and prevents nonenzymatic ADP-ribosylation | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + D-ribose 5-phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00011 | - |
ADP-ribose | mutant enzyme E82Q, in presence of Mg2+ | Thermus thermophilus | |
0.00014 | - |
ADP-ribose | mutant enzyme E82Q, in presence of Zn2+ | Thermus thermophilus | |
0.00015 | - |
ADP-ribose | mutant enzyme E86Q, in presence of Mg2+ | Thermus thermophilus | |
0.00032 | - |
ADP-ribose | mutant enzyme E86Q, in presence of Zn2+ | Thermus thermophilus | |
0.7 | - |
ADP-ribose | mutant enzyme D126N, in presence of Mg2+ | Thermus thermophilus | |
0.7 | - |
ADP-ribose | mutant enzyme E129Q, in presence of Mg2+ | Thermus thermophilus | |
2 | 8 | ADP-ribose | wild-type enzyme, in presence of Zn2+ | Thermus thermophilus | |
2.2 | - |
ADP-ribose | mutant enzyme D128N, in presence of Mg2+ | Thermus thermophilus | |
4.1 | - |
ADP-ribose | mutant enzyme E127Q, in presence of Mg2+ | Thermus thermophilus | |
5.7 | - |
ADP-ribose | wild-type enzyme, in presence of Mg2+ | Thermus thermophilus |